Polysaccharide extracts of Cirsium japonicum protect rat H9c2 myocardial cells from oxidative stress induced by hydrogen peroxide

在传统中医体系中,大蓟被广泛用于治疗胃病、肺部疾病和心血管疾病.本研究旨在探讨大蓟多糖提取物对过氧化氢（H2O2）诱导的大鼠心肌细胞损伤的保护作用.大蓟多糖经水提取,乙醇沉淀;然后通过高效液相色谱法和红外光谱进行分析.大鼠心肌细胞经H2O2处理建立细胞损伤模型.MTT法检测结果表明,大蓟多糖可明显改善H2O2引起的心肌细胞损伤并呈剂量依赖性.此外,大蓟多糖可明显抑制H2O2诱导的心肌细胞凋亡,减少caspase-3,caspase-8,和caspase-9蛋白的表达.此外,p38丝裂原活化蛋白激酶信号通路可能参与了大蓟多糖对心肌细胞的保护作用.大蓟多糖对过氧化氢引起的大鼠心肌细胞的氧化应激反应具有保护作用.

Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects of Daji （Cirsiumjaponicum） polysaccharide extracts （CJP） against hydrogen peroxide （H2O2） shock in rat H9c2 myocardial cells. First,CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquidchromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cellinjury model. The 3- （4,5- dimethylthiazol- 2-yl）-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatmentsignificantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. Inaddition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP onmyocardial cells. Therefore, we conclude that polysaccharide extracts of Daji （Cirsium japonicum） protect rat H9c2myocardial cells from oxidative stress induced by H2O2.