摘要
Objective： To evaluate the regulatory effects of Banxia （Pinellia ternata, P） and Huanglian （Coptis chinensis, C） drugpair, derived from Banxiaxiexin soup, on the gastrointestinal movement of mice with functional dyspepsia. Methods：Mice were treated with different proportions of the P and C drug pair （1：1, 3：1, and 4：1） for 10 days, and subsequentlyinjected with atropine （ATR） or neostigmine （NEO）. The effects of the different proportions of P and C were evaluatedbased on the alvine advance rate. In addition, we used the same modeling method used in the first experiment andadministered P： C at ratio of 3：1 and at different doses respectively （4.68 g/L, 2.34 g/L, and 1.17 g/L）, and tested levelsof the gastrointestinal hormones, gastrin （GAS）, vasoactive intestinal polypeptide （VIP）, and somatostatin （SS） in thesmall intestinal tissue using an enzyme-linked immunosorbent assay. Results： In the groups of NEO-induced mice, P：Cat ratios of 1：1, 3：1, and 4：1 significantly reduced the alvine advance rate compared with the NEO model group （P =0.003, P = 0.012 and P = 0.021, respectively）. In the groups of ATR-induced mice, only P：1 at ratio of 3：1 significantlyincreased the alvine advance rate compared with the ATR model group （P = 0.007）. After exposure to P： C at ratio of 3：1and at different dose, the GAS level was lower in the low-, medium-, and high-dose NEO groups than that in the NEOmodel group （P = 0.001, P = 0.004, and P = 0.003, respectively）. The VIP levels were higher in the medium-andhigh-dose NEO groups than that in the NEO model group （P = 0.004 and P = 0.002, respectively）. In addition, the SSlevel increased in the NEO medium-dose group compared with that in the NEO model group （P = 0.002）. The GAS levelwas higher in the ATR medium- and high-dose groups than in the ATR model group （P = 0.007 and P = 0.021,respectively）. The VIP level was lower in the ATR low-, medium-, and high-dose than that in the ATR model group （P =0.001, P = 0.001, and P = 0.001, respectively）. Furthermore, the SS level was lower in the ATR medium- and high-dosegroups than that in the ATR model group （P = 0.001 and P = 0.006）. Conclusion： The PC drug pair bidirectionallyadjusted the NEO- and ATR-induced functional dyspepsia in mice by modulating GAS, VIP, and SS levels in theintestine.
摘要目的：观察半夏-黄连药对对功能性消化不良小鼠胃肠运动的调节作用.方法：采用三种不同配比的半夏-黄连药液（1：1,3：1,4：1）予昆明种雄性小鼠灌胃.10天后用阿托品腹腔注射法复制胃肠运动抑制模型,新斯的明腹腔注射法复制胃肠运动亢进模型,并且检测其小肠推进率.此外,使用与第一个实验相同的建模方法,对半夏-黄连3：1配比组设立高、中、低三种剂量浓度（4.68g/L,2.34g/L和1.17g/L）.运用酶联免疫吸附试验检测小肠组织内胃肠激素如胃泌素、血管活性肠肽和生长抑素的含量.结果：在新斯的明诱导的胃肠运动亢进模型中,3种不同配比的半夏-黄连药液（1：1,3：1,4：1）均能显著降低小鼠的小肠推进率（P=0.003,P=0.012,P=0.021）;在阿托品诱导的胃肠运动抑制模型组中,仅3：1配比的半夏-黄连药液能显著增加模型小鼠的小肠推进率.在新斯的明诱导的胃肠运动亢进模型中,半夏-黄连药液低剂量组、中剂量组和高剂量组的胃泌素水平均低于模型组（P=0.001,P=0.004,P=0.003）.半夏-黄连药液中剂量组和高剂量组的血管活性肠肽水平均高于模型组（P=0.004,P=0.002）.半夏-黄连药液中剂量组的生长抑素水平高于模型组（P=0.002）;在阿托品诱导的胃肠运动抑制模型中,半夏-黄连药液中剂量组和高剂量组的胃泌素水平均高于模型组（P=0.007,P=0.021）.半夏-黄连药液低剂量组,中剂量组和高剂量组的血管活性肠肽水平均高于模型组（P=0.001,P=0.001,P=0.001）.半夏-黄连药液中剂量组的生长抑素水平低于模型组（P=0.001和P=0.006）.结论：半夏-黄连可以通过影响小肠组织内的胃泌素、血管活性肠肽和生长抑素的水平来双向调节由阿托品及新斯诱导的小鼠功能性消化不良.
