基于类石墨相碳三氮四纳米片与砷的电子转移效应的亚砷酸根检测方法

Electrochemiluminescence assay for arsenite based on electron transfer interaction between g-C_3N_4 and arsenite

建立了一种基于类石墨相碳三氮四纳米片(g-C_3N_4 NSs)与砷之间的电子转移效应的亚砷酸根电致化学发光(ECL)检测方法。g-C_3N_4在S2O2-8共反应剂中发射强的阴极ECL信号。将富含G/T碱基的DNA((GT)19)组装到金纳米粒子修饰的g-C_3N_4(Au-g-C_3N_4)电极表面,(GT)19可以特异性吸附亚砷酸根,并且其DNA结构发生改变,从而拉近亚砷酸根与Au-g-C_3N_4之间的距离。g-C_3N_4和亚砷酸根之间发生电子转移,进而使g-C_3N_4的ECL强度降低。据此建立了亚砷酸根检测方法,线性范围为0.1~50mg·m-3,检测限为0.07mg·m-3,对实际水样进行加标回收实验,结果表明该方法具有很高的实用性。

Herein,a novel electrochemiluminescence（ECL）biosensor based on electron transfer between g-C3N4 and arsenite（As（Ⅲ））is used to detect arsenite.In the present of S2 O2-8,g-C3N4 can emit strong cathodic ECL signal.（GT）19 ssDNA is assembled on the gold nanoparticles functionalized g-C3N4（Au-gC3N4）modified electrode surface.（GT）19 ssDNA can specifically adsorb arsenite and thereby captures the arsenite on the ssDNA/Au-g-C3N4）modified electrode surface.So the distance between As（Ⅲ）and Au-gC3N4 is shortened.Electron transfer between g-C3N4 and As（Ⅲ）decreases the ECL intensity of g-C3N4.The linear range of the ECL method for As（Ⅲ）detection is 0.1-50 mg·m-3,with the detection limit of0.07 mg·m-3.This method can also be used for detection of As（Ⅲ）in environmental water samples.