Effect of splitting combination of different components of Zhilong Huoxue Tongyu Capsule on vascular remodeling in hypertension

目的：观察蛭龙活血通瘀胶囊拆方组分对大鼠血管NF-κB-P65、ICAM-1表达的影响,探讨蛭龙活血通瘀胶囊及其拆方组分对高血压血管重构的影响及作用机制.方法：手术组缩窄左肾动脉建立肾性高血压大鼠模型.分别给予相应药物1次/日灌胃,以同体积生理盐水灌胃正常组、假手术组与模型组.灌胃4周后,取大鼠胸主动脉,依次固定、包埋、切片、HE染色,免疫组化的方法检测血管NF-κB-p65.结果：1.药物对高血压大鼠胸主动脉NF-κB蛋白表达的影响（免疫组化法）：电镜下,棕黄色颗粒为NF-κB蛋白阳性表达,主要分布于血管中膜平滑肌.正常组及假手术组可见少量的棕黄色颗粒;模型组可见散在分布的大量表达棕黄色颗粒;经治疗后,卡托普利组、全方组表达较模型组明显减少,补气组、温通经络组、活血化瘀组表达较模型组有所减少.其中卡托普利组较全方组有所减少;补气组较活血化瘀组表达明显减少,与温通经络组表达相当.结论：蛭龙活血通瘀胶囊其对肾性高血压大鼠血管重构机制,可能与其降低血压、抗氧化应激、抗炎作用有关.各组方配伍后产生了显著的协同增效作用,其配伍后体现了中医药多靶点的治疗优势.

Objective： To observe the effects of the components of Zhilong Huoxue Tongyu Capsule （ZLHXTY） on the expression of NF-κB-P65 and ICAM-1 in blood vessels of rats, and to explore the effect and mechanism of ZLHXTY and its ingredients on vascular remodeling in hypertension. Methods： A rat model of renal hypertension was established by narrowing the left renal artery in the operation group. The corresponding drugs were given once a day respectively. The Normal group, Sham-operation group and Model group were administered with the same volume of normal saline. After 4 weeks of gavage, the thoracic aorta of rat was taken, followed by fixation, embedding, sectioning and HE staining, and NF-κB -p65 was detected by immunohistochemistry. Results： 1. The effect of drugs on the expression of NF-κB in thoracic aorta of hypertensive rats （immunohistochemistry）： A small amount of NF-κB protein was expressed in the Normal group and the Sham-operate group. The NF-κB expression in the Model group was significantly increased, and the electron microscope image showed that the brown-yellow granule was distributed in the vascular smooth muscle of the membrane. After treatment, the expression of NF-κB in the Captopril group and the Whole prescription group was significantly reduced compared with the Model group, while the protein expression in other groups was decreased compared with the Model group. The protein expression of captopril group was decreased compared with that of the Whole prescription group. Compared with Promoting blood circulation for removing blood stasis group, the expression of NF-κB in Benefiting qi group was significantly decreased and was similar to that in Warming meridian group. Conclusion： The vascular remodeling mechanism of ZLHXTY on renal hypertension rats may be related to its effects on lowering blood pressure, antioxidant stress and anti-inflammation. After the compatibility of formula produced a significant synergistic effect, and its compatibility after embodies multiple targets for treatment of advantages of traditional Chinese medicine.