摘要
[Objectives] To systematically identify and analyze pharmacognostical features of Mongolian medicine Shoushen and Naishoushen( dairy tablets) to provide scientific basis for the establishment and identification of its quality standard. [Methods] According to relevant methods specified in Appendix to 2010 Chinese Pharmacopoeia,the water content,total ash,and extracts of Shoushen and Naishoushen were detected,and thin layer chromatography( TLC) was applied to make qualitative identification. Gastrodin was used as the reference substance,extracted with 70% methanol,and then sprayed with ethyl acetate-formic acid-water( 9∶ 1∶ 0. 2) as the developing solvent,and then sprayed with 10% phosphomolybdic acid ethanol solution and heated at 105℃ to clear spot color. UV-Visible spectrophotometry and high performance liquid chromatography( HPLC) were used to analyze the content of polysaccharides and gastrodins in Shoushen and Naishoushen. The chromatographic column was a ZOBB AX eclipse XDB-C_(18)( 4. 6 mm × 250 mm,5 cm) column with the methanol-0. 04% phosphoric acid solution( 8∶92) as the mobile phase,the flow rate of 1. 0mL/min,the detection wavelength of 222 nm,and the column temperature of 30℃.[Results]In the thin-layer chromatography test,spots of the same color appeared at the positions corresponding to the chromatogram of the reference substance. Gastrodin eluted under high pressure reached baseline separation. Gastrodin had a good linearity in the concentration range of 0. 009-0. 09 mg/m L. The regression equation was Y = 586 866 X + 425 821( R^2= 0. 999 6),and the average recovery rate was100. 1%. Precision test,reproducibility test,and stability test conformed to the requirements. The results of extracts of three batches of samples were 26. 13%-42. 58%,water content was 3. 47%-5. 31%,and total ash was 5. 43%-6. 33%. [Conclusions] The quality control method has high reliability,high sensitivity,high specificity,high accuracy,and high stability. The results are expected to provide a scientific basis for the identification,resource utilization,and improvement of quality standard for Shoushen and Naishoushen.
[Objectives] To systematically identify and analyze pharmacognostical features of Mongolian medicine Shoushen and Naishoushen （dairy tablets） to provide scientific basis for the establishment and identification of its quality standard.[Methods] According to relevant methods specified in Appendix to 2010 Chinese Pharmacopoeia , the water content, total ash, and extracts of Shoushen and Naishoushen were detected, and thin layer chromatography （TLC） was applied to make qualitative identification. Gastrodin was used as the reference substance, extracted with 70% methanol, and then sprayed with ethyl acetate-formic acid-water （9∶ 1∶ 0.2） as the developing solvent, and then sprayed with 10% phosphomolybdic acid ethanol solution and heated at 105℃ to clear spot color. UV-Visible spectrophotometry and high performance liquid chromatography （HPLC） were used to analyze the content of polysaccharides and gastrodins in Shoushen and Naishoushen. The chromatographic column was a ZOBB AX eclipse XDB-C 18 （4.6 mm × 250 mm, 5 cm） column with the methanol-0.04% phosphoric acid solution （8∶ 92） as the mobile phase, the flow rate of 1.0 mL/min, the detection wavelength of 222 nm, and the column temperature of 30℃.[Results] In the thin-layer chromatography test, spots of the same color appeared at the positions corresponding to the chromatogram of the reference substance. Gastrodin eluted under high pressure reached baseline separation. Gastrodin had a good linearity in the concentration range of 0.009-0.09 mg/mL. The regression equation was Y = 586 866 X ＋ 425 821 （ R 2 = 0.999 6）, and the average recovery rate was 100.1%. Precision test, reproducibility test, and stability test conformed to the requirements. The results of extracts of three batches of samples were 26.13%-42.58%, water content was 3.47%-5.31%, and total ash was 5.43%-6.33%.[Conclusions] The quality control method has high reliability, high sensitivity, high specificity, high accuracy, and high stability. The results are expected to provide a scientific basis for the identification, resource utilization, and improvement of quality standard for Shoushen and Naishoushen.
基金
Supported by Inner Mongolia Science and Technology Foundation Project(kjt15sf22)
