血清对Ⅱ型脊髓灰质炎病毒感染性滴度检测结果的影响

Influence on Type Ⅱ Poliovirus of Infectious Titers of Serum

目的比较6种不同血清对Ⅱ型脊髓灰质炎病毒感染性滴度(CCID50)检测结果的影响。方法分别采用6种未处理血清和6种预处理血清组(免疫吸附法)进行Ⅱ型脊髓灰质炎病毒感染性滴度检测(微细胞病变法),检测了不同组血清中Ⅱ型脊髓灰质炎病毒中和抗体滴度,并使用处理血清组进行促细胞生长试验。最后分析不同血清对感染性滴度的影响。结果6种未处理血清组病毒感染性滴度检测结果差异有统计学意义(P<0.01),6种处理血清组病毒感染性滴度差异无统计学意义(P>0.05)。未处理组2号血清的中和抗体滴度为1∶4,6号血清的中和抗体滴度为1∶8,其余各组血清的中和抗体滴度均<1∶4。6种处理血清组的中和抗体滴度均为1∶2。血清促细胞生长试验显示6种处理组血清细胞生长趋势正常。结论Ⅱ型脊髓灰质炎病毒感染性滴度检测前使用免疫吸附对血清进行预处理,可降低血清中存在的中和抗体对检测的影响,为比较不同实验室数据提供可能。

Objective To compare 6 different serum＇ s influence on type Ⅱ poliovirus tissue culture median infective dose（CCID50）.Methods We detected the neutral antibody titer of type Ⅱ poliovirus using 6 original serum and 6 predisposed serum（immunoadsorption method）.At the same time,we also performed cell growth experiment using predisposed serum.In addition,we analyzed the data to find out different serum＇s influence on type Ⅱ poliovirus CCID50.Results There is a significant difference among the type Ⅱpoliovirus CCID50 results of the 6 original serum groups.However,an opposite result had been shown in those predisposed serum groups.In addition,we noticed that the neutral antibody titer of type Ⅱ poliovirus in original serum groups is 1∶ 2 except No.2 group with 1∶ 4.The No.6 predisposed serum group had a 1∶ 8 neutral antibody titer but the other 5 predisposed serum groups share a 1∶ 2 neutral antibody titer.Furthermore,these cells had a normal growth tendency in the 6 predisposed serum groups.Conclusion The neutralizing antibody＇s influence on type Ⅱ poliovirus CCID50 test can be reduced by predisposing serum through immunoadsorption method,which provides the possibility for different laboratories＇ data comparison.