幽门螺杆菌感染致胃黏膜及尿中RNA损伤水平测定

Determination of the Level of RNA Damage in Gastric Mucosa and Urine in Patients with Helicobacter Pylori Infection

目的检测幽门螺杆菌(Hp)感染患者胃窦部黏膜及尿液中8-氧化脱氧鸟苷及8-氧化鸟苷水平,探讨Hp感染与DNA及RNA氧化损伤的相关性,并比较不同取材部位水平的差异。方法 Hp阳性组85例,Hp阴性组31例。分别留取尿液标本以层析分析法,同时两组中各30例取胃窦部黏膜以免疫组化法检测8-氧化脱氧鸟苷及8-氧化鸟苷。结果胃窦部黏膜检测结果显示Hp阳性组8氧化鸟苷高于Hp阴性组,差异有统计学意义(P<0.05),8-氧化脱氧鸟苷高于Hp阴性组,差异无统计学意义(P>0.05);尿液检测,Hp阳性组8-氧化鸟苷及8-氧化脱氧鸟苷水平均高于Hp阴性组,差异均有统计学意义(P<0.05)。结论尿液检测与胃黏膜组织检测结果趋势一致,Hp感染后DNA及RNA氧化损伤水平高于Hp阴性对照组,且尿液检测更敏感。RNA氧化产物8-氧化鸟苷可能是Hp感染致病的一个较敏感的指标。

Objective To explore the correlation and difference of damage to DNA and RNA with helicobacter pylori by detecting the level of 8-oxod Gsn and 8-oxo Gsn of urine and gastric mucosa in patients infected by helicobacter pylori.Methods A total of 85 patients were involved in Hp positive group and 31 patients in Hp negative group.8-oxo Gsn and 8-oxod Gsn were detected in urine sample by chromatographic analysis,and gastric sinus mucosa by immunohistochemical method.Results The consequence of gastric sinus mucosa detection presented that 8-oxo Gsn was higher in Hp positive group than that in HP negative group and the difference was statistically significant（P〈 0.05）.8-oxod Gsn was higher in Hp positive group than that in Hp negative group,but the difference was not statistically significant（P〉 0.05）.Urine tests showed that both 8-oxo Gsn and 8-oxod Gsn were higher in Hp positive group than that in Hp negative group,and the differences were statistically significant（P〈 0.05）.Conclusion The detection results of urine were similar with that of gastric mucosa and urine tests seemed to be more sensitive.The oxidative damage of DNA and RNA was higher in patients with Hp infection.8-oxo Gsn as one of the RNA oxidative damage products may be a sensitive indicator of Hp infection.