VDR基因多态性与结直肠癌发病风险的相关性

Relationship Between Vitamin D Receptor Gene Polymorphism and the Risk of Colorectal Cancer

目的研究维生素D受体(vitamin D receptor,VDR)基因多态性与结直肠癌的发病风险之间的相关性。方法随机选择2013年5月~2016年8月笔者医院收治的350例结直肠癌患者作为研究组,另招募350例笔者医院门诊处健康的体检者作为对照组。抽取所有受试者外周血后抽提基因组DNA,选择29个VDR单核苷酸多态性(SNPs)进行基因分型,并测量血浆维生素D浓度。采用Logistic回归模型评估结直肠癌的优势比(OR)和95%置信区间(CI)。结果 rs2254210、rs1540339、rs2107301、rs11168267、rs11574113、rs731236、rs3847987和rs11574143等8个VDR的SNP与结肠直肠癌的风险相关(P<0.05)。在血浆维生素D浓度较高的人中rs11574113、rs3847987和rs11574143这3个SNP位点与结肠直肠癌的风险相关性更显著。rs7968585位点不同血浆维生素D水平下各位点的基因型患者的比例间的差异有统计学意义(P<0.05)。结论维生素D受体基因多态性与结直肠癌发病风险具有一定相关性,开展维生素D基因多态性检测对于预测结直肠癌的发生并指导临床用药具有一定的意义。

Objective To study the correlation between vitamin D receptor（VDR） gene polymorphism and the risk of colorectal cancer.Methods Three hundred and fifty patients with colorectal cancer treated in our hospital from May 2013 to August 2016 were randomly selected as the study group,and 350 healthy subjects were recruited in this study.The genomic DNA was extracted from peripheral blood of all subjects.Twenty-nine VDR single nucleotide polymorphisms（SNPs） were selected and genotyped,and the plasma vitamin D concentration was measured.Logistic regression model was used to evaluate the odds ratio（OR） and 95% confidence interval（CI） of colorectal cancer.Results Rs2254210,rs1540339,rs2107301,rs11168267,rs11574113,rs731236,rs3847987 and rs11574143 VDR SNPs were associated with the risk of colorectal cancer（P〈 0.05）,and the risk of colorectal cancer was significantly higher than that of colorectal cancer（P 〈0.05）.The SNPs of rs11574113,rs3847987 and rs11574143 were more correlated with the risk of colorectal cancer in people with higher plasma vitamin D concentrations.There was a significant difference in the proportion of patients with genotype at different levels of plasma vitamin D in the rs7968585 locus（P〈 0.05）.Conclusion The polymorphism of vitamin D receptor gene has a certain correlation with the risk of colorectal cancer,and the detection of vitamin D gene polymorphism has a certain significance for predicting the occurrence of colorectal cancer and guiding clinical medicine.