来源湛江红树内生真菌的二氢异香豆素及其抑菌活性

Dihydroisocoumarin Compounds from Endophytic Fungi of Rhizophora apiculata and Its Antibacterial Activity

为了研究湛江红树(Rhizophora apiculata)植物内生真菌Fusarium sp.F67和B42的二氢异香豆素类次级代谢产物及其抑菌活性,采用色谱技术对菌株F67和B42的代谢产物进行分离纯化,确认所得化合物的结构为:3,6,8-三羟基-3,4,5,7-四甲基-3,4-二氢异香豆素(sclerotinin A)(1),二氢橘霉素(2)、5-甲基蜂蜜曲菌素(3)、5-羧基蜂蜜曲菌素(4)、4-羟基蜂蜜曲菌素(5)和蜂蜜曲菌素(6),并利用滤纸片琼脂扩散法检测化合物对7种常见致病菌的抑菌活性。在1.0 g/L质量浓度下化合物1~4对受试菌株的抑菌圈范围为5.1~11.0 mm,其中化合物4的抑菌活性相对较强,抑菌谱相对较广,可为挖掘新型抗菌药物的研究提供补充。

In order to study the secondary metabolites of dihydroisocoumarin compounds from mangrove endophytic fungi Fusarium sp. F67 and B42,the metabolites of strains F67 and B42 were isolated and purified by chromatographic techniques,and their structures were determined as follows： 3,6,8-trihydroxy-3,4,5,7-tetramethyl-3,4-dihydroisocoumarin（ sclerotinin A）（ 1）,dihydrocitrinone（ 2）,5-methylmellein（ 3）,5-carboxymellein（ 4）,4-hydroxymellein（ 5） and mellein（ 6）. Antibacterial activities of compounds 1 ~ 4 in1. 0 g/L show that the inhibitory zone against the tested strains ranges from 5. 1 to 11. 0 mm. Among them,compound 4 has the strongest antibacterial activities and the broadest bacteriostatic spectra. These results provide supplement to the research and development of new antibacterial drug.