产焦性没食子酸菌株筛选及其培养条件优化

Screening and Optimization of Culture Condition of a Pyrogallol-producing Strain

研究了肠杆菌、短乳杆菌和植物乳杆菌3种菌种催化底物没食子酸降解产焦性没食子酸的能力,其中产焦性没食子酸效果较好的是肠杆菌和植物乳杆菌。对不同温度、底物浓度、pH值、离子浓度等反应条件对肠杆菌和植物乳杆菌没食子酸脱羧酶酶活的影响进行分析,并确定了这两株菌降解没食子酸的培养条件。结果表明:在培养温度为28℃、没食子酸浓度为30 mmol/L,(NH_4)_2SO_4浓度8 mmol/L,MgSO_4浓度为80 mmol/L,初始培养基pH值6.0,培养48 h时,肠杆菌催化没食子酸产焦性没食子酸58.11%;在培养温度为37℃、底物浓度为10 mmol/L,(NH_4)_2SO_4浓度为8 mmol/L,MgSO_4浓度为20 mmol/L,初始培养基pH值6.5,培养48 h时,植物乳杆菌催化没食子酸产焦性没食子酸83.31%。

Three bacteria strains including Enterobacter spp.,Lactobacillus brevis and Lactobacillus plantarum were tested for degrading gallic acid into pyrogallol. The factors affecting the activity of gallate decarboxylase from E. spp. and L. plantarum,including substrate concentration, temperature, pH, magnesium sulfate（ MgSO4） concentration were also studied. The experiment results showed that the yield of pyrogallol by E. spp. could reach 58. 11% with the temperature 28 ℃,30 mmol/L gallic acid,8 mmol/L（ NH4）2SO4,80 mmol/L MgSO4 and pH 6.0 after cultured for 48 h. Meanwhile,the yield of pyrogallol by L. plantarum could reach 83.31% with the temperature 37 ℃,substrate concentration 10 mmol/L,concentration of（ NH4）2SO48 mmol/L,concentration of MgSO420 mmol/L and pH 6.5 after cultured for 48 h.