蓝光对小鼠角膜上皮和角膜全层厚度的影响——基于光学相干断层扫描血管造影观察

Effects of blue light on the thickness of corneal epithelium and full-thickness of the cornea in mice by optical coherence tomography angiography

目的应用光学相干断层扫描血管造影(optical coherence tomography angiography,OCTA)观察蓝光对小鼠角膜上皮和角膜全层厚度的影响。方法选取40只小鼠随机分为实验组和对照组,每组各20只,其中实验组小鼠每天8~16 h于蓝光环境下饲养,而对照组小鼠于正常环境下饲养。分别于照射前及照射后1周、2周、1个月、2个月、3个月,利用OCTA分别测量实验组和对照组小鼠的角膜上皮和角膜全层厚度。结果与蓝光照射前相比,实验组、对照组分别在照射后1周、2周、1个月,各区域角膜上皮厚度无明显改变,差异均无统计学意义(均为P>0.05)。与蓝光照射前相比,对照组在照射后2个月、3个月各区域角膜上皮厚度无明显改变,差异均无统计学意义(均为P>0.05)。与对照组相比,实验组角膜上皮中央、N5、I5、T5区域明显增厚,差异均有统计学意义(均为P<0.05);蓝光照射后3个月,与对照组相比,实验组小鼠角膜中央、内环及N6、T6区域角膜上皮厚度均明显增厚,差异均有统计学意义(均为P<0.05)。蓝光照射前,照射后1周、2周、1个月、2个月、3个月,实验组与对照组相比,各区域角膜全层厚度比较无明显改变,差异均无统计学意义(均为P>0.05)。两组角膜上皮厚度的极值,即最大值和最小值差值比较,差异有统计学意义(P<0.05),但两组角膜全层厚度最小值和最大值的差值比较无明显统计学意义(P>0.05)。结论蓝光能引起小鼠角膜上皮厚度改变,且中央区域改变较为明显,但短期内角膜全层厚度无明显改变。

Objective To investigate the effects of blue light on the thickness of corneal epithelium and full-thickness of the cornea in mice by optical coherence tomography angiography（OCTA）. Methods Totally 40 mice were collected and randomly divided into experimental group and control group,with 20 mice in each group,and the experimental mice were raised in the blue light environment from 8 to 16 hours per day,while the controls were reared in normal environment. Then the thickness of corneal epithelium and full-thickness of the cornea in both groups were measured by OCTA before irradiation and one week,two weeks,one month,two months and three months after irradiation,respectively. Results Compared with pre-irradiation,the thickness of corneal epithelium of all regions did not change significantly in both groups at 1 week,2 weeks,and 1 month after irradiation,and the differences were not statistically significant（ all P〈0. 05）.C ompared with before irradiation,the corneal epithelium thickness of the control group at 2 months and 3 months after irradiation did not change significantly,and there was no significant difference（both P〈0. 05）. Compared with the control group,the corneal epithelium at central,nasal 5 mm,inferior 5 mm,and temporal 5 mm regions in the experimental group were significantly thickened,and the differences were statistically significant（ all P〈0. 05）. Three months after irradiation,compared with the control group,the thickness of corneal epithelium in the central and inner regions of the cornea and nasal 6 mm and temporal 6 mm regions of the experimental group were significantly thickened,and the differences were statistically significant（ all P〈0. 05）. There was no significant change in the corneal full thickness betw een the experimental group and the control group before irradiation and 1 week,2 weeks,1 month,2 months,and 3 months after irradiation,and the differences were not statistically significant（ all P〈0. 05）. Furthermore,the difference in the extremum value of corneal epithelial thickness,namely the maximum and the minimum,was significantly different in both groups（ P〈0. 05）,but the difference in the extremum value of the full-thickness of the cornea was not significant in the two groups（ P 0.05）. Conclusion The blue light can change the thickness of corneal epithelium in mice,and the change of the central region is obvious,but the fullthickness of the cornea do not significantly change in a short term.