甘肃山楂与山里红总黄酮的提取及含量测定方法研究

Studies on Extract and Determination of Total Flavonoids in C. kansuensis and Crataegus pinnatifida

建立了甘肃山楂与山里红总黄酮的提取及含量的测定方法,并优选其总黄酮的最佳提取条件。采用紫外-可见分光光度法,以芦丁为对照品,硝酸铝显色反应,在507 nm处测定甘肃山楂与山里红总黄酮含量;并采用正交试验的方法对甘肃山楂与山里红总黄酮提取效果的诸因素(乙醇浓度、料液比、提取时间等)进行优选。结果显示:芦丁浓度在0.0082~0.0820 mg/m L范围内线性关系良好,r=0.9996,加样回收率RSD=3.42%;乙醇体积分数60%、料液比1∶40、提取时间3 h、提取次数3次,提取效果最好。该方法的提取率高,精密度高、重现性良好,加样回收率和准确度高,为甘肃山楂与山里红总黄酮含量测定及优化提取工艺提供了参考依据。

To establish a method for extracting and determining the total flavonoids in C.kansuensis and Crataegus pinnatifida , and study the extracting technology condition of the flavonoids . The flavonoids was determined with Rutin as standard and aluminium nitrate as developer by UV spectrophotomety in 507 nm. The factors of extraction effect （such as ethanol concentration,solid-liquid ratio,extracting time） are preferred by orthogonal test for C.kansuensis and Crataegus pinnatifida . The linear ranges for rutin were 0.0082~0.0820 mg/mL（ r =0.9996）. The average recovery RSD was 3.42%.When ethanol concentration was 60% , solid-liquid ratio was 1∶40, extracting time was 3 h, the extraction times was 3, the extact effect was the best. This method is reliable, sensitive, reproducible, high recovery, accurate. It can provide the references for extracting and determining the total flavonoids of C.kansuensis and Crataegus pinnatifida .