柠檬酸对小鼠脂肪细胞甘油三酯及其代谢产物含量的影响

Effects of Citric Acid on Contents of Triglyceride and Its Metabolites of Mouse Adipocytes

本试验旨在研究柠檬酸对小鼠脂肪细胞甘油三酯(TG)及其代谢产物含量的影响。将小鼠3T3-L1前脂细胞诱导成成熟的脂肪细胞作为研究对象,在诱导培养第14天,加入含0(对照组)、20(试验Ⅰ组)、50(试验Ⅱ组)、200μmol/L的柠檬酸完全培养基(试验Ⅲ组)培养细胞,于0、36、72 h收集细胞检测TG合成和分解代谢中关键限速酶及代谢产物的含量。结果表明:在36和72 h,试验Ⅰ、Ⅱ、Ⅲ组TG含量显著高于对照组(P<0.05);各试验组醛缩酶(FDA)、乙酰辅酶A羧化酶(ACC)和脂肪酸合成酶(FAS)含量均显著或极显著高于对照组(P<0.05或P<0.01);各试验组激素敏感脂肪酶(HSL)、肉碱脂酰转移酶1(CPT1)、游离脂肪酸(FFA)及乙酰辅酶A含量均极显著低于对照组(P<0.01)。结果提示,柠檬酸能够促进小鼠脂肪细胞TG的合成和沉积,能显著或极显著提高TG合成代谢中FDA、ACC、FAS产物的含量,极显著降低TG分解代谢中HSL、CPT1、FFA及乙酰辅酶A产物的含量,20μmol/L为最佳剂量。

The purpose of this study was to investigate the effects of citric acid on contents of triglyceride and its metabolites of mouse adipocytes. Firstly inoculated the mouse 3T3-L1 cells into mature adipocytes as the test subject, the cells were cultured in complete mediums containing 0 （CK group）, 20 （test group Ⅰ）, 50 （test group Ⅱ）and 200 μmol/L citric acid （test group Ⅲ） on the 14th day, respectively. The cells were collected at 0, 36 and 72 h for the detection of key rate-limiting enzyme contents in TG synthesis and catabolism, and TG metabolite contents, respectively. The results showed as follows： at 36 an 72 h, TG content in test groups Ⅰ, Ⅱ and Ⅲ was significantly higher than that in CK group（ P 〈0.05）. The contents of aldolase （FDA）, acetyl-CoA carboxylase （ACC） and fatty acid synthetase （FAS）in test groups were significantly higher than those in CK group（ P 〈0.05 or P 〈0.01）. The contents of hormone-sensitive lipase （HSL）, carnitine palmityl transferase 1 （CPT1）, free fatty acids （FFA） and acetyl CoA in test groups were significantly lower than those in control group（ P 〈0.01）. In conclusion, citric acid can promote the synthesis and deposition of TG of mouse adipocytes, significantly increase the contents of FDA, ACC,and FAS in TG synthesis, and significantly reduce the contents of HSL, CPT1, FFA, acetyl CoA in TG disintegration; the optimal dosage of citric acid is 20 μmol/L.