摘要
[Objectives] To study the anti-inflammatory effects and its possible action mechanism of Pereskia aculeate Miller on rats with adjuvant arthritis( AJ). [Methods] Fifty SD rats( half male and half female) were randomly divided into 5 groups: blank group,model group,positive control group( Glucosidorum Tripterygll Totorum( GTT),12 mg/kg),and P. aculeate high and low dose group( 0. 86 and0. 43 g/m L). Except the blank group,other groups were induced with complete Freund's adjuvant( CFA) to establish the AA rat model. On the 17 th day of modeling,the drug was externally applied for soaking,and the diameter of foot hole and foot joint before and after administration was measured to observe the degree of swelling. The enzyme-linked immunosorbent assay( ELISA) was adopted to determine the inflammatory cytokines interleukin-1β( IL-1β) and tumor necrosis factor-α( TNF-α). [Results] Compared with the model group,the degree of swelling of the foot sole and foot joints was reduced in the P. aculeate high dose group and the positive control group( P < 0. 05). According to ELISA test,compared with the model control group,the serum levels of IL-1β( P < 0. 05) and TNF-α( P < 0. 05) were significantly reduced in the P. aculeate high dose group and the positive control group. Compared with the positive control group,there was no significant difference( P > 0. 05). [Conclusions] P. aculeate has significant inhibitory effects on foot swelling of adjuvant arthritis in rats,and the action mechanism is possibly related to the decrease of IL-1β and TNF-α.
[Objectives] To study the anti-inflammatory effects and its possible action mechanism of Pereskia aculeate Miller on rats with adjuvant arthritis（ AJ）. [Methods] Fifty SD rats（ half male and half female） were randomly divided into 5 groups： blank group,model group,positive control group（ Glucosidorum Tripterygll Totorum（ GTT）,12 mg/kg）,and P. aculeate high and low dose group（ 0. 86 and0. 43 g/m L）. Except the blank group,other groups were induced with complete Freund＇s adjuvant（ CFA） to establish the AA rat model. On the 17 th day of modeling,the drug was externally applied for soaking,and the diameter of foot hole and foot joint before and after administration was measured to observe the degree of swelling. The enzyme-linked immunosorbent assay（ ELISA） was adopted to determine the inflammatory cytokines interleukin-1β（ IL-1β） and tumor necrosis factor-α（ TNF-α）. [Results] Compared with the model group,the degree of swelling of the foot sole and foot joints was reduced in the P. aculeate high dose group and the positive control group（ P 〈 0. 05）. According to ELISA test,compared with the model control group,the serum levels of IL-1β（ P 〈 0. 05） and TNF-α（ P 〈 0. 05） were significantly reduced in the P. aculeate high dose group and the positive control group. Compared with the positive control group,there was no significant difference（ P 〉 0. 05）. [Conclusions] P. aculeate has significant inhibitory effects on foot swelling of adjuvant arthritis in rats,and the action mechanism is possibly related to the decrease of IL-1β and TNF-α.
基金
Supported by Natural Science Foundation Project of Guangxi(2017GXNSF AA198255)
Young Scholar Project of Natural Science Foundation of Guangxi University of Chinese Medicine(2015LX037)
Student's Platform for Innovation and Entrepreneurship Training Program of Guangxi Zhuang Autonomous Region,China in 2016(201610601016)
