Determination of Nine Phenolic Components in Leaves of Crataegus pinnatifida Bge.

[Objectives] To determine the nine phenolic components in the leaves of Crataegus pinnatifida Bge. [Methods] The reversedphase high-performance liquid chromatography( RP-HPLC) was applied. [Results] Nine phenolic components showed a good linear relationship in the range of 2-500 μg/m L with r in the range of 0. 999 5-0. 999 9. The recovery rate of spiked samples ranged from 93. 7% to110. 2%,and the relative standard deviation was in the range of 0. 69%-4. 58%. The leaves of 29 cultivars of C. pinnatifida Bge. were measured,and the average content of the nine phenolic components was as follows: isoquercitrin,hyperoside,procyanidin C1,procyanidin D1,epicatechin,procyanidin B2,chlorogenic acid,eucomic acid,and vitexin 2 "-O-rhamnoside. The contents of flavonoids and phenolic acids were high,up to 15 mg/g D. W,and the content of procyanidins was up to 6 mg/g D. W. [Conclusions]This method is easy and accurate in determination of phenolic components in the leaves of C. pinnatifida Bge.

[ Objectives] To determine the nine phenolic components in the leaves of Crataegus pinnatifida Bge. [ Methods ] The reversedphase high-performance liquid chromatography （RP-HPLC） was applied. [ Results] Nine phenolic components showed a good linear relationship in the range of 2 - 500 μg/mL with r in the range of 0. 999 5 - 0. 999 9. The recovel7 rate of spiked samples ranged from 93.7% to 110.2% , and the relative standard deviation was in the range of 0.69% -4.58%. The leaves of 29 cultivars of C. pinnatifida Bge. were measured, and the average content of the nine phenolic components was as follows ： isoquercitrin, hyperoside, procyanidin C1, procyanidin D1, epicatechin, procyanidin B2, chlorogenic acid, euconfic acid, and vitexin 2＂ -O-rhanmoside. The contents of flavonoids and phenolic acids were high, up to 15 mg/g D. W, and the content of procyanidins was up to 6 mg/g D.W. [ Conclusions] This method is easy and accu- rate in determination of phenolic components in the leaves of C. pinnatifida Bge.