前列腺癌患者癌组织中miR-21表达水平及与预后的关系分析

The expression level of miR-21 in patients with prostate cancer and its relationship with the prognosis

目的分析前列腺癌患者癌组织中miRNA-21(miR-21)表达水平及与预后的关系。方法回顾性分析2011年1月—2012年6月四川大学华西医院76例前列腺癌行手术切除患者资料,采用特异性TaqMan探针实时定量PCR法检测患者切除前列腺癌组织及癌旁前列腺组织miR-21表达水平,分析其与患者预后的关系。结果 miR-21在前列腺癌组织中相对表达量为(2.68±0.54),明显高于癌旁组织的(1.14±0.42)(t=19.62,P<0.05);前列腺癌患者miR-21高表达者57例(75%),低表达者19例(25%),2组TNM分期、Gleason评分、复发转移比较,差异均有统计学意义(P<0.05),而年龄、前列腺体积、血清前列腺特异抗原水平比较,差异无统计学意义(P>0.05);miR-21表达量低组平均生存期32.5个月,miR-21表达量高组平均生存期24.8个月,经Kaplan-Meier生存分析显示,2组差异有统计学意义(P=0.019)。结论 miR-21在前列腺癌患者癌组织中具有高表达现象,对前列腺癌的诊断及预后评价具有指导意义。

Objective To analyze the expression level of miRNA-21（ miR-21） in cancer tissues of patients with prostate cancer and its relationship with the prognosis. Methods The data of76 patients with prostate cancer undergoing surgical resection in the West China Hospital of Sichuan University from January 2011 to June 2012 were analyzed retrospectively. The expression levels of miR-21 in cancer tissues and paracancerous normal prostate tissues were detected by specific Taq Man probe real-time quantitative PCR method,and the relationship with the prognosis was analyzed. Results The relative expression of miR-21 in prostate cancer tissues was significantly higher than that in adjacent tissues [（ 2. 68±0. 54） vs（ 1. 14±0. 42） ]（ t = 19. 62,P〈0. 05）. MiR-21 was highly expressed in 57 cases（ 75%） and lowly expressed in 19 cases（ 25%）. There were significant differences between the two groups in TNM stage,Gleason score,recurrence and metastasis（ P〈0. 05）,while there were no significant differencs in age,prostate volume and serum prostate-specific antigen level（ P〉0. 05）. The average survival time of group with low expression level of miR-21 and group with high expression level of miR-21 was 32. 5 months and 24. 8 months,respectively. The Kaplan-Meier survival analysis showed that the difference between the two groups was significant（ P = 0. 019）.Conclusion miR-21 is highly expressed in prostate cancer tissues,which can indicate the prognosis.