响应面法优化超声波辅助提取正红菇色素工艺

Optimization of Ultrasonic-assisted Extraction Technology of Pigment from Russula vinosa linolbl by Response Surface Methodology

以正红菇为原料,采用超声波辅助提取正红菇色素,以正红菇色素溶液的吸光度为指标,通过单因素实验,考察了乙醇浓度、提取时间、提取温度和料液比对正红菇色素提取液吸光度的影响,并结合响应面实验对提取工艺进行优化。结果显示,超声波辅助提取正红菇色素的最佳工艺条件为:乙醇浓度55%,提取时间29 min,提取温度68℃,料液比1∶35 g/m L。在最佳工艺参数条件下,实验提取的正红菇色素的吸光度值为0.404,与理论预测值0.406相近,表明该色素提取的工艺条件是合理可行的。

The pigment of Russula vinosa linolbl was thaken as material and extracted by ultrasonic-assisted extraction.The effects of variables on extraction yields of Russula vinosct linolbl pigment, such as ethanol concentration, liquid- solid ratio, extraction temperature and extraction time were investigated by single factor experiment.The response surface methodology was used to optimize the extraction conditions. Results showed that the optimum extraction conditions were as follows： ethanol concentration of 55%, ultrasonic time 29 min, extraction temperature 68 ℃ and a material- liquid ratio of 1 ： 35 g/mL. The predicted absorbance of pigment extracts under the optimized conditions was 0.404, which was similar to the theoretical prediction value 0.406. Thus, the optimized pigment extraction conditions were reliable and feasible.