摘要
目的观察转录因子GATA3对Kaposi肉瘤相关疱疹病毒(Kaposi's sarcoma-associated herpesvirus,KSHV)感染细胞增殖和凋亡的影响。方法培养KSHV感染细胞i SLK-BAC与KSHV未感染细胞i SLK-Puro,采用实时定量PCR(real-time PCR)法和Western blot检测GATA3的表达情况;分别用CCK8法和流式细胞术检测过表达GATA3后细胞的增殖能力与凋亡水平。结果 GATA3在i SLK-BAC细胞中的mRNA和蛋白表达水平均低于其在i SLK-Puro细胞中的表达水平;过表达GATA3后i SLK-BAC细胞增殖能力下降,凋亡率升高。结论转录因子GATA3能够抑制KSHV感染细胞系i SLK-BAC的增殖,并促进其凋亡。
Objective To observe the effects of transcription factor GATA3 on the proliferation and apoptosis in Kaposi's sarcoma-associated herpesvirus(KSHV)infected cells. Methods KSHV infected cells iSLK-BAC and KSHV uninfected cells iSLK-Puro were cultured,and the expression of GATA3 was detected by real-time PCR and Western blot. Using CCK8 assay and flow cytometry to detect the proliferative ability and apoptosis level after GATA3 plasmid was transfected into iSLK-BAC cells.Results The expression level of GATA3 mRNA and protein in iSLK-BAC cells was lower than that in iSLK-Puro cells. After GATA3 plasmid was transfected into iSLK-BAC cells,the proliferative ability was remarkably inhibited and the apoptosis rate increased.Conclusion Transcription factor GATA3 could inhibit the proliferation of KSHVinfected iSLK-BAC cells and promote apoptosis.
作者
崔梦
郑军
李之启
王发展
王可楹
郑志红
徐江
曾妍
CUI Meng1, ZHENG Jun2, LI Zhiqi1, WANG Fazhan1, WANG Keying1, ZHENG Zhihong3, XU Jiang2, ZENG Yan1(1.Department of Biochemistry,Key Laboratory of Xinjiang Endemic and Ethnic Disease,School of Medicine,Shihezi University,Shihezi 832000,China; 2. Department of Stomatology,the First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi 832000,China; 3. Functional Experimental Center,School of Medicine,Shihezi University,Shihezi 832000,China)
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2018年第8期863-867,共5页
The Chinese Journal of Dermatovenereology
基金
国家自然科学基金(U1603117
81560473)