HPLC法测定金莲花汤中绿原酸、咖啡酸、葛根素、牡荆素、迷迭香酸

Content assay of five components in Jinlianhua decoction by HPLC

目的建立金莲花汤中绿原酸、咖啡酸、葛根素、牡荆素、迷迭香酸的HPLC含量测定方法,并对采用不同来源的药材制备的3批金莲花汤中的上述5种成分进行含量测定。方法采用HPLC法,色谱柱为Synergi Polar-RP苯基C18色谱柱(250 mm34.6 mm,4μm);流动相为1%乙酸(A)~乙腈(B);洗脱程序(0~30 min,5%~14%B;30~40 min,14%~14%B;40~42 min,14%~19%B;42~60 min,19%~25%B;60~63 min,25%~5%B;63~64 min,5%~5%B);柱温35℃;检测波长286 nm;进样量10μL。结果测定3批金莲花汤中绿原酸、咖啡酸、葛根素、牡荆素、迷迭香酸含量分别为:0.34、0.08、9.73、0.93、0.32 mg/g;0.13、0.03、8.37、0.33、0.21 mg/g;0.37、0.07、8.36、0.12、0.26 mg/g。结论该方法精密度、稳定性、重复性良好,适用于金莲花汤中上述5种成分的含量测定;用不同来源的药材制备的金莲花汤中上述5种成分的含量存在差异,其中葛根素的含量最高。

Objective To establish an HPLC method for content assay of chlorogenic acid, caffeic acid, puerarin, vitexin and rosmarinic acid in Jinlianhua decoction, and the contents of the above five components were determined in three batches of Jinlianhua decoction prepared from the drugs bought from different places of China. Methods The analysis was performed on a Synergi Polar-RP Phenomenex C18 column（250 mm × 4.6 mm, 4 μm） using 1% acetic acid solution（A） – acetonitrile（B） as the mobile phases with gradient elution（0-30 min, 5%-14% B; 30-40 min, 14%-14% B; 40-42 min, 14%-19% B; 42-60 min, 19%-25% B; 60-63 min, 25%-5% B; 63-64 min, 5%-5%B）. The column temperature was set at 35℃, the UV detection wavelength was 286 nm, and the injection volume was 10 μL. Results The contents of chlorogenic acid, caffeic acid, puerarin, vitexin and rosmarinic acid in the three batches of Jinlianhua decoction were determined as follows： 0.34, 0.08, 9.73, 0.93, 0.32 mg/g; 0.13, 0.03, 8.37, 0.33, 0.21 mg/g; 0.37, 0.07, 8.36, 0.12, 0.26 mg/g. Conclusion The method is precise, stable and reproducible. It is suitable for the determination of chlorogenic acid, caffeic acid, puerarin, vitexin and rosmarinic acid in Jinlianhua decoction. The content of these fives components is different in the Jinlianhua decoctions prepared from the drugs purchased from different places of China, and that of puerarin is the highest.