新疆伊犁州艾滋病治疗患者耐药分析

Drug resistance analysis of AIDS patients in Yili Prefecture,Xinjiang

目的了解新疆伊犁州抗病毒治疗患者耐药状况。方法选择HIV在治患者病毒载量>1 000拷贝/ml的样本1 137份,HIV-1基因型耐药检测方法,用in-house套式聚合酶链反应扩增HIV pol区基因,用Chromas Pro软件进行序列拼接分析,进入Stanford HIV耐药数据库序列结果比对,获得耐药位点和耐药程度分析结果。结果 1 137例患者中有399例对任何药物都未产生耐药,至少对一种蛋白酶抑制剂耐药的人数占6.16%;至少对一种核苷类逆转录酶抑制剂耐药的人数占23.39%;至少对一种非核苷类逆转录酶抑制剂耐药的人数占44.86%。结论及时检测HIV-1耐药突变,不间断地对患者进行服药依从教育尤为重要。

Objective To understand the drug resistance status of patients for antiviral therapy in Yili Prefecture, Xinjiang. Methods 1137 samples were selected from HIV patients with viral load greater than 1000 copies/ml. In the detection of HIV-1 genotype resistance, in-house nested polymerase chain reaction was used to amplify the HIV pol region gene, using ChromasPro software for sequence splicing and analysis, compared with the sequence results of Stanford HIV drug resistance database, and the drug resistance sites and drug resistance analysis results were obtained. Results Of the 1137 patients, 399 patients were resistant to any drug, and 6.16% patients were resistant to at least one protease inhibitor; 23.39% patients were resistant to at least one nucleoside reverse transeriptase inhibitor; 44.86% of patients were resistant to at least one non-nucleo-side reverse transcriptase inhibitor. Conclusion It is especially important to timely test HIV-1 resistance mutations and to continue to take medication adherence to patients.