七氟醚对神经发育期大鼠远期认知功能影响及机制探讨

The long-term cognitive function and mechanism of sevoflurane in neurodevelopmental rats

目的:探讨七氟醚对神经发育期大鼠远期认知功能影响及机制。方法:选择清洁级SD雄性大鼠96只,根据随机数字表法,分为空白组、七氟醚1组及七氟醚2组,空白组吸入空气,七氟醚1组吸入2.8%七氟醚2 h,2组吸入2.8%七氟醚4 h,30 d后3组均行水迷宫实验,对比3组大鼠的认知功能。实验结束后,用Western blot法检测大鼠的海马脑源性神经营养因子(BDNF)、突触后致密物质95(PSD-95)及突触蛋白-1水平。结果:七氟醚1组第2、3、4、5天的逃避潜伏期均明显高于空白组,穿越原平台位置次数、平台所在象限滞留时间均明显低于空白组,而七氟醚2组第2、3、4、5天的逃避潜伏期均明显高于空白组及七氟醚1组,穿越原平台位置次数、平台所在象限滞留时间均明显低于空白组及七氟醚1组(P<0.05)。七氟醚1组的BDNF、PSD-95、突触蛋白-1明显低于空白组;七氟醚2组的BDNF、PSD-95、突触蛋白-1明显低于空白组及七氟醚1组(P<0.05)。结论:七氟醚可能通过降低BDNF、PSD-95、突触蛋白-1水平,抑制海马神经元突触可塑性来影响大鼠的远期认知功能,影响程度随麻醉时间延长而增加。

Objective：To investigate the long-term cognitive function and mechanism of sevoflurane in neurodevelopmental rats.Methods：96 male SD rats with clean grade were divided into the blank group,sevoflurane 1 group and sevoflurane 2 group,the blank group were snifting,sevoflurane 1 group were inhaled 2. 8% sevoflurane for 2 h,sevoflurane 2 group were given 2. 8% sevoflurane for4 h,the rats were given Morris water maze after 30 days,the cognitive function of rats in three groups were compared. After experiment,the hippocampal brain-derived neurotrophic factor（ BDNF）,postsynaptic dense matter 95（ PSD-95） and synaptic protein-1 level were tested by Western blot. Results：The escape latency of sevoflurane 1 group in 2,3,4 and 5 d were higher than blank group,the crossing the original platform time and the position of the platform were lower than blank group. While the escape latency of sevoflurane 2 group were higher than blank group and sevoflurane 1 group,the crossing the original platform time and the position of the platform were lower than blank group and sevoflurane 1 group（ P〈0. 05）. The BDNF,PSD-95 and synaptic protein-1 of sevoflurane 1 group were lower than blank group,which in sevoflurane 2 group were lower than blank group and sevoflurane 1 group（ P〈0. 05）. Conclusion：Sevoflurane can decrease the BDNF,PSD-95 and synaptic protein-1 level,inhibit the synaptic plasticity of hippocampal neurons to affect the long-term cognitive function,and the influence degree are increase with the duration of anesthesia.