落叶松林型对其针叶内几种防御蛋白活力和次生代谢物含量的影响

Effects of Forest Type on Activity of Several Defense Proteins and Contents of Secondary Metabolites in Larch Needles

[目的]为明确落叶松林型对其化学防御能力的影响。[方法]本实验以长白落叶松纯林及长白落叶松-水曲柳4∶4、2∶10带状混交林为研究对象,分析不同林型下落叶松针叶内几种防御蛋白活力及次生代谢化合物含量的变化。[结果]表明:30年生(HJ30(4∶4))和20年生(HJ20(4∶4))落叶松与水曲柳4∶4带状混交林中,落叶松针叶内几种防御蛋白活力和次生代谢物含量均显著高于纯林(P<0.05);在HJ20(4∶4)中,落叶松针叶中PPO、PAL、TI活力及黄酮和木质素含量最高,且PPO、PAL活性和木质素含量显著高于20年生(HJ20(2∶10))落叶松与水曲柳2∶10带状混交林(P<0.05);在HJ20(2∶10)中,落叶松针叶内CI活性显著高于HJ20(4∶4)(P<0.05),单宁含量也高于HJ20(4∶4),但差异不显著(P>0.05)。[结论]试验发现,长白落叶松-水曲柳带状混交林成林显著增强落叶松针叶内防御蛋白活力、增加次生代谢物的含量,从而提高了长白落叶松自身抗虫性;且HJ20(4∶4)对落叶松的诱导防御能力强于HJ20(2∶10),HJ20(4∶4)混交方式比HJ20(2∶10)更适用于混交林营造。

[Objective]To illuminate the effects of the forest type on chemical defense of pure and mixed larch stand. [Method] Larch（Larix olgensis） forest and larch-ash（Fraxinus mandschurica） mixed forest were used as the test materials,and the activity of several defense proteins and the content of secondary metabolic compounds were tested in larch needles of different forest type. [Result]The results showed that the activity of several defense proteins and the content of secondary metabolites in larch needles of 30-year-old larch-ash mixed by strips of 4 ∶ 4（HJ30（4∶4）） and 20-year-old larch-ash mixed by strips of 4∶4（HJ20（4∶4）） significantly increased,compared with that of 30-year-old L. olgensis pure stand（CL30） and 20-year-old L. olgensis pure stand（CL20）（P〈0. 05）. It was found in Zhangjiawan site that the activities of polyphenol oxidase（PPO）,phenylalanine ammonia-lyase（PAL）,trypsin inhibitor（TI） and the contents of flavonoids and lignin in larch needles of HJ20（4∶4）were the highest,while the activities of PPO,PAL and the content of lignin were higher than that in larch needles of 20-year-oldlarch-ash mixed by strips of 2∶10（HJ20（2∶10））（P〈0. 05）. In larch needles of HJ20（2∶10）,the CI activity significantly increased（P〈0. 05）,and the tannin content added（P〉0. 05） in contrast to that in needles of HJ20（4∶4）.[Conclusion]These results indicate that larch-ash mixed forests significantly strengthen the activity of defense proteins and increase the content of secondary metabolites in larch needles,which improve the resistance to defoliators.Compared with HJ20（2∶10）,the induced defense of HJ20（4∶4）is stronger,and HJ20（4∶4）is more applicable to build the mixed forest.