摘要
[Objectives] This study was conducted to improve the genetic character of Epipremnum aureum and provide a new way for genetic breeding of E. aureum. [Methods] In the paper,protoplasts were isolated from tender leaves of E. aureum and Scindapsus aureus var. wilcoxii by using 1% cellulase and 1% peetinase. Then the protoplasts were purified and dynamically identified,and fusion of protoplasts of E. aureum and S. aureus was induced by PEG. [Results]A large quantity of protoplasts were obtained from tender leaves of E. aureum and S. aureus successfully,and the activity of protoplasts was over 90%. Fusion product was obtained by PEG induction,and the fusion rate was 16. 3%. [Conclusions]Protoplasts were isolated from tender leaves of E. aureum and S. aureus successfully,and a stable fusion system was built by PEG induction.
[Objectives] This study was conducted to improve the genetic character of Epipremnum aureum and provide a new way for genetic breeding of E. aureum. [Methods] In the paper,protoplasts were isolated from tender leaves of E. aureum and Scindapsus aureus var. wilcoxii by using 1% cellulase and 1% peetinase. Then the protoplasts were purified and dynamically identified,and fusion of protoplasts of E. aureum and S. aureus was induced by PEG. [Results]A large quantity of protoplasts were obtained from tender leaves of E. aureum and S. aureus successfully,and the activity of protoplasts was over 90%. Fusion product was obtained by PEG induction,and the fusion rate was 16. 3%. [Conclusions]Protoplasts were isolated from tender leaves of E. aureum and S. aureus successfully,and a stable fusion system was built by PEG induction.
