长叶红砂RtMYB1基因的克隆及表达

Cloning and expression analysis of RtMYB1 gene from Reaumuria trigyna

MYB转录因子家族在植物抵抗非生物胁迫过程中发挥着重要的调控作用。基于转录组测序数据,从珍稀泌盐盐生植物长叶红砂(Reaumuria trigyna)中克隆一个MYB转录因子基因,命名为RtMYB1。RtMYB1基因具有780bp的ORF(open reading frame)序列,编码236个氨基酸残基组成的蛋白。RtMYB1能够被盐、冷、高温、紫外照射(UV)和干旱(PEG)5种非生物胁迫诱导表达,这表明RtMYB1基因可能参与长叶红砂响应非生物胁迫的调节途径。本研究为深入了解长叶红砂的抗逆机理及发掘优异的抗逆基因奠定了基础。

The MYB transcription factor family plays an important regulatory role in imparting resistance to abiotic stress in plants.Based on transcriptome sequencing data,an MYB transcription factor was cloned from a rare secretohalophyte,Reaumuria trigyna,and named RtMYB1.The RtMYB1 gene has an ORF of 780 bp,encoding 236 amino acid residues.Expression of the RtMYB1 gene can be induced by stress factors,including salt,cold,high temperature,ultraviolet radiation（UV）,and drought（PEG）,indicating that the gene may be involved in regulating the response of R.trigyna to abiotic stress.This study lays the foundations to explore and better understand the resistance mechanisms against abiotic stress in R.trigyna.