新型多光子成像技术研究进展

Advances in multiphoton microscopy technologies

相比于传统的光学成像技术,近年来获得快速发展的新型多光子成像技术具有穿透深度大,组织光损伤小,信噪比高,且可方便进行光学层析成像的特点,故而被广泛应用于包括脑、肿瘤、胚胎在内的多种活体组织成像中。本综述回顾了新型多光子成像技术的诞生与发展历程,包括微型化双光子成像技术、双光子内窥技术和三光子成像技术,概括分析了其基本原理与成像特点,讨论了这一领域具有代表性的最新研究成果,重点总结了其在生物学基础研究领域和临床医学诊断中的主要应用,并展望了其未来的应用与发展前景。可以预见,随着激光器和光探测技术的不断进步,多光子成像技术将会得到更大的发展与更加广泛的应用。

Compared with traditional optical imaging techniques,the fast-developing multiphoton microscopy technologies possess multiple advantages,such as deep penetration,low tissue photo-damaging,high signalto-noise ratio,and excellent optical sectioning ability.Therefore,they have been widely applied in tissue-level microscopy in vivo for brains,tumors and embryos.This article reviews the recent development of new multiphoton microscopy technologies,including miniaturized two-photon microscopy,two-photon endoscopy,and three-photon microscopy.The review briefly illustrates their principles and characteristics,introduces the latest progresses in these areas,summarizes their main applications in basic research and clinical diagnosis,and discusses their potential application and development in the future.With the advances in laser devices and opticaldetectors,multiphoton microscopy will become an important tool for biomedical research with broad applications.