添加Se元素对Cu污染土壤磷酸酶活性的影响

Effects of Se Addition on the Acid Phospatase Activities in Cu Polluted Soils

通过对Cu污染土壤添加Se元素的土培试验,研究了不同加Se水平和培养时间对不同浓度Cu污染土壤酶活性的影响。结果表明,重金属Cu显著抑制土壤磷酸酶活性,加入适量Se元素可提高Cu污染土壤酶活性,土壤磷酸酶活性与Se浓度呈显著或极显著相关。Se对土壤磷酸酶活性影响随培养时间而变化,培养25 d、45 d土壤酶活性增加,到45 d增幅最大,之后培养65 d有所下降,但仍高于培养5 d的土壤磷酸酶活性。由此可得,Cu污染土壤中添加Se元素可以提高土壤磷酸酶活性,降低Cu对土壤磷酸酶活性的抑制作用。土壤Cu浓度在35~1 600 mg/kg范围,最适加Se范围为1.0~2.0 mg/kg,最佳培养时间约为45 d。

Through incubation experiments by Se addition in Cu polluted soil,the effects of different Se concentrations and incubation time on acid phosphatase activities in the soil polluted by different Cu concentrations were studied. Results showed that Cu stress suppressed acid phosphatase activities significantly,while Se addition improved the acid phosphatase activities in the Cu polluted soil and the soil acid phosphatase activities were significantly related to Cu concentration. The soil acid phosphatas activities varied with the incubation time. The enzyme activities increased on the 25 th,45 th day,had the largest increase on the 45 th day and then declined on the 65 th but were still higher than that on the 5 th day. In conclusion,Se addition in Cu polluted soil could increase acid phosphatase activities in Cu pollution soil,diminished the suppressed effects of Cu on acid phosphatase activities. During the 35~ 1600 mg/kg Cu concentration,in order to improve the soil acid phosphatase activities,the most appropriate Se addition concentration was from 1.0 to 2.0 mg/kg,and the best incubation time was 45 days.