摘要
【目的】利用复合益生菌(植物乳杆菌植物亚种Zhang-LL和副干酪乳杆菌KL1)发酵复方中草药,考察发酵过程中的影响因素,确定发酵工艺参数。【方法】Zhang-LL和KL1菌株经活化、扩增后发酵复方中草药,从发酵液提取多糖。通过单因素多水平试验(复方中药发酵温度、培养基初始pH、接种量、发酵时间)和正交试验设计,以苯酚-硫酸法测定发酵后多糖含量和得率,确定最优条件。【结果】结果表明,最佳发酵工艺条件为初始pH5.0,接种量5%,发酵温度34℃,时间72h。在此优化条件下,发酵复方中药液的多糖得率为1.13%,是优化前的1.65倍,未发酵的2.51倍。
【Objective】The combined probiotics(Lactobacillus plantarum subsp.plantarum zhang-LL and Lactobacillus paracasei KL1)were used to ferment mixtured Chinese herbal medicine.【Method】Zhang-LL and KL1 strains were activated and amplified to ferment mixtured herbs and polysaccharides from fermented broth were extracted and measured.The fermentation temperature,initial pH,inoculation amount and fermentation time were investigated.Single factor multi level test and orthogonal test were applied to determine the best fermentation conditions.【Result】The results showed that the best indicators were initial pH 5.0,inoculation amount 5%,incubation temperature 34℃,fermentation time 72 h.【Conclusion】The polysaccharide yield reached 1.13%in the conditions above,1.65 times of initial conditions or 2.51 times of non-fermentation.
作者
李嘉懿
张红星
谢远红
熊利霞
高秀芝
刘慧
连正兴
LI Jiayi, ZHANG Hongxing, XIE Yuanhong, XIONG Lixia, Gao Xiuzhi, LIU Hui , LIAN Zhengxing(l.CFood Science and Engineering College, Beijing University of Agriculture, Beijing Laboratory of Food Quality and Safety, Beijing engineering laboratory of key technology development of Microecologics, Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products, Beijing 102206, China;2. College of Animal Science & Technology, China Agricultural University, Beijing 100094, Chin)
出处
《北京农学院学报》
2018年第3期83-87,共5页
Journal of Beijing University of Agriculture
基金
转基因生物新品种培育重大专项(2016ZX08008-03)
关键词
植物乳杆菌植物亚种
副干酪乳杆菌
复方中草药
发酵
多糖
Lactobacillus plantarum subsp. plantarum Lactobacillus paracasei
Chinese herbal medicine
fermentation
polysaccharide