益生菌发酵复方中草药产多糖的工艺优化

Optimization of fermentation for producing polysaccharides from Chinese herbal by combined probiotics

【目的】利用复合益生菌(植物乳杆菌植物亚种Zhang-LL和副干酪乳杆菌KL1)发酵复方中草药,考察发酵过程中的影响因素,确定发酵工艺参数。【方法】Zhang-LL和KL1菌株经活化、扩增后发酵复方中草药,从发酵液提取多糖。通过单因素多水平试验(复方中药发酵温度、培养基初始pH、接种量、发酵时间)和正交试验设计,以苯酚-硫酸法测定发酵后多糖含量和得率,确定最优条件。【结果】结果表明,最佳发酵工艺条件为初始pH5.0,接种量5%,发酵温度34℃,时间72h。在此优化条件下,发酵复方中药液的多糖得率为1.13%,是优化前的1.65倍,未发酵的2.51倍。

【Objective】The combined probiotics（Lactobacillus plantarum subsp.plantarum zhang-LL and Lactobacillus paracasei KL1）were used to ferment mixtured Chinese herbal medicine.【Method】Zhang-LL and KL1 strains were activated and amplified to ferment mixtured herbs and polysaccharides from fermented broth were extracted and measured.The fermentation temperature,initial pH,inoculation amount and fermentation time were investigated.Single factor multi level test and orthogonal test were applied to determine the best fermentation conditions.【Result】The results showed that the best indicators were initial pH 5.0,inoculation amount 5%,incubation temperature 34℃,fermentation time 72 h.【Conclusion】The polysaccharide yield reached 1.13%in the conditions above,1.65 times of initial conditions or 2.51 times of non-fermentation.