基于UPLC最佳色谱参数的连翘中7个活性成分一测多评分析方法的建立及应用

A New QAMS Analytical Method for Rapid Determination of Seven Bioactive Components in Forsythia suspensa Based on UPLC

目的:建立连翘中7个活性成分的含量测定方法。方法:对UPLC色谱参数进行优化,建立一测多评分析方法,并对该方法的线性范围、回收率、精密度、稳定性、重复性进行考察。结果:选用乙腈-0.1%磷酸水作为流动相,以277 nm作为检测波长,最佳柱温为30℃,最佳流速为0.3 m L/min。绿原酸、咖啡酸、芦丁、金丝桃苷、连翘酯苷A、连翘苷和牛蒡子苷元分别在0.009 80~0.196 00μg(r=0.998 4),0.008 96~0.179 20μg(r=0.998 9),0.008 00~0.240 00μg(r=0.999 4),0.009 16~0.183 20μg(r=0.999 2),0.020 72~0.414 40μg(r=0.999 5),0.019 82~0.396 40μg(r=0.999 1),0.006 96~0.139 20μg(r=0.999 2)范围内线性关系良好,平均加样回收率分别为95.4%(RSD=3.78%),96.3%(RSD=4.61%),97.2%(RSD=4.04%),102.6%(RSD=3.91%),103.7%(RSD=2.37%),98.5%(RSD=1.99%),95.1%(RSD=4.58%)。结论:建立了连翘中7个活性成分含量测定的一测多评分析方法,具有较好的灵敏性和准确性。

Objective： To establish a method to determine the content of seven bioactive components in Forsythia suspensa.Methods： The chromatographic parameters of UPLC were optimized. A QMAS analytical method was built, with its linear range,recovery rate, precision, stability and repeatability evaluated. Results： The mobile phase consisted of 0.1% phosphoric acid solution and acetonitrile. The detection wavelength was 277 nm. The optimum column temperature was 30 ℃ and the optimum speed was 0.3 m L/min.Chlorogenic acid, caffeic acid, rutin, hyperoside, forsythoside A, phillyrin and arctigenin showed good linear relationships within the ranges of 0.009 80~0.196 00 μg（r=0.998 4）, 0.008 96~0.179 20 μg（r=0.998 9）, 0.008 00~0.240 00 μg（r=0.999 4）, 0.009 16~0.183 20 μg（r=0.999 2）, 0.020 72~0.414 40 μg（r=0.999 5）, 0.019 82~0.396 40 μg（r=0.999 1）, 0.006 96~0.139 20 μg（r=0.999 2）,and the average recoveries were 95.4%（RSD=3.78%）, 96.3%（RSD=4.61%）, 97.2%（RSD=4.04%）, 102.6%（RSD=3.91%）,103.7%（RSD=2.37%）, 98.5%（RSD=1.99%）, 95.1%（RSD=4.58%）, respectively. Conclusion： A QMAS analytical method for seven bioactive components in Forsythia suspensa has been established, which has a high sensitivity and accuracy.