坪山区从业人员携带沙门菌的血清型分布及药敏试验分析

Analysis of Salmonella serotype distribution and drug sensitivity of practitioners in Pingshan District

目的了解深圳市坪山区从业人员携带沙门菌的血清学分布和耐药特点,为有效控制沙门菌引起的公共卫生事件及控制耐药菌株传播提供理论依据。方法收集2015年7-11月肛拭子标本4 620份,对培养出的可疑菌株进行生化鉴定和血清学分型,鉴定结果为沙门菌时再进行常规药敏试验检测。结果共筛查出61例带菌者,阳性率为1.32%。61株沙门菌分属5个群,其中B群最多,共31株(50.82%),其次是C1群12株(19.67%);血清学分型排名前3位的分别是斯坦利沙门菌(13.12%)、德比沙门菌(11.48%)及鼠伤寒沙门菌(9.84%)。61株沙门菌药敏试验结果显示,对复方磺胺甲噁唑耐药率最高,为27.9%,其次为哌拉西林、替卡西林及替卡西林/克拉维酸,均为24.6%,对头孢噻肟、头孢他啶、头孢吡肟、美罗培南及亚胺培南全部敏感。结论坪山地区从业人员携带的沙门菌以B群为主,呈多样性分布。药敏试验结果显示部分沙门菌对多种抗菌药物耐药,临床治疗首选头孢类药物,同时应加强常规抗菌药物监测,指导携带者规范用药治疗,防止食源性疾病发生。

Objective To analyze the serological distribution and drug resistance of Salmonella carried by practitioners in Pingshan District,and to provide a theoretical basis for effective control of salmonella-induced public health events and drug-resistant strains.Methods A total of 4 620 samples of anal swabs were collected from July 2015 to November 2015 in Pingshan district.The biochemical identification and serotyping of the cultured suspicious strains were carried out,and then identified Salmonella would be used for drug susceptibility test.Results A total of 61 cases were detected,the positive rate was 1.32%.A total of 61 strains of Salmonella were divided into five groups,among which 31（50.82%）were in B group which was the most,followed by 12（19.67%）in C1 group;the first three most serotypes were Salmonella tumefaciens（13.12%）,Salmonella terding（11.48%）and Salmonella typhimurium（9.84%）.The results of drug sensitivity analysis of 61 Salmonella strains showed that the highest resistance rate was caused by compound sulfamethoxazole（27.9%）,followed by piperacillin,ticarcillin and ticarcillin/clavulanic acid（all were 24.6%）.Thiadiazole,ceftazidime,cefepime,metoprolide and imipenem were all showed sensitive.Conclusion Salmonella,which is carried by practitioners in Pingshan area,is mainly distributed in B group.Drug sensitivity results showed that some strains of Salmonella were resistant to a variety of antibiotics.Cephalosporins are the first choice for clinical treatment.Meanwhile,monitoring the use of conventional antibiotics should be strengthened to guide the carriers to standardize their medication,and prevent food borne disease.