补髓生血冲剂对慢性再生障碍性贫血患者造血调控因子表达水平的影响

The influence of Soluble granules of Bu Sui Sheng xue on IL-1β、IFN-γ、TNF-α expression level of chronic aplastic anemia patients

目的 :探讨补髓生血冲剂对慢性再生障碍性贫血 (CAA)患者造血调控因子IL - 1β、IFN -γ、TNF -α表达水平的影响。方法 :采用现代医学梅联免疫 (ELISA)测定方法和放射免疫 (RIA)方法 ,分别对使用该冲剂治疗前后的CAA患者血清学IL - 1β、IFN -γ、TNF -α水平进行检测 ,并与对照组及正常组作比较。结果 :疗前CAA患者血清IL - 1β、IFN -γ、TNF -α表达水平与正常组相比有显著性差异 (P <0 0 1) ;经补髓生血冲剂治疗后 ,其血清学水平有所恢复 ,且实验组与对照组相比差异显著 (P <0 0 5 )。结论 :补髓生血冲剂可使CAA患者血清中IL - 1β水平提高 ,IFN -γ、TNF -α水平降低 ,从而改善紊乱的免疫状态 ,使造血细胞增殖、分化功能在一定程度上得以恢复。

Objective:To observe the influence of Soluble granules of Bu Sui Sheng xue on IL-1β、IFN-γ、TNF-α expression level of chronic aplastic anemia(CAA) patients. Methods:By means of advanced modern medical experiments enzyme linked immunosorbent assay(ELISA) and radioimmunoassay(RIA),we detected the level of IL-1β、IFN-γ、TNF-α in blood serum of CAA patients before and after treatment with Soluble granules of Bu Sui Sheng xue,and compared with control group and normal group. Results:The levels of IL-1β、IFN-γ、TNF-α of CAA patients have remarkable difference compared with normal group(P<0 01). After the treatment with Soluble granules of Bu Sui Sheng xue,there is remarkable difference in the levels between treatment group and control group(P<0 05). Conclusion:The Soluble granules of Bu Sui Sheng xue can enhance the IL-1β level,decrease the IFN-γ、TNF-α level,and adjust the disturbance of immune function.