重症肺炎患儿支气管肺泡灌洗液中IL-6、IL-10、IL-17及HMGB1水平

Study on Levels of IL-6,IL-10,IL-17 and HMGB1 in the Bronchoalbeolar Lavage Fluid of Children with Severe Pneumonia

目的:探讨重症肺炎患儿支气管肺泡灌洗液(BALF)中IL-6、IL-10、IL-17及高迁移率族蛋白B1(HMGB1)水平与病情严重程度及病程的关系。方法:选取住院治疗并行支气管肺泡灌洗术的重症肺炎患儿100例,将胸部CT提示受累肺叶≥2/3设定为A组、肺叶受累<2/3设定为B组,根据病程再将A、B两组分别分为A1组和B1组(急性期患儿,病程≤7 d)、A2组和B2组(恢复期患儿,病程>7 d);ELISA法检测BALF中IL-6、IL-10、IL-17及HMGB1水平,比较各组上述炎症因子水平。结果:A组IL-17水平高于B组,差异有统计学意义(P<0.05);、A1和B1组IL-6、IL-17水平高于A2和B2组,差异有统计学意义(P<0.05);A1组IL-6水平高于B1组,差异有统计学意义(P<0.05)。结论:BALF中IL-6、IL-17可作为反映急性期重症肺炎患儿病情严重程度的有用指标。

Objective： To explore the relationship between the level of IL-6,IL-10,IL-17 and HMGB1 in the bronchoalveolar lavage fluid（ BALF） and severity and course of the disease. Methods：A total of 100 cases of severe pneumonia children treated with bronchoalveolar lavage were divided into group A（ pulmonary lobe involvement no less than 2/3） and group B（ pulmonary lobe involvement less than 2/3） according to chest CT. Then according to the course,group A and group B were further divided into two groups ： group A1 and group B1（ children with acute phase,course ≤7 d）,group A2 and B2（ children with recovery period,course 〉7 d）. The levels of IL-6,IL-10,IL-17 and HMGB1 in BALF were detected by Elisa,and the levels of the above mentioned inflammatory factors in each group were compared. Results： The ILl-17 level of group A was higher than that of group B,and the difference was statistically significant（ P〈0. 05）. The levels of IL-6 and IL-17 of group A1 and B1 were higher than those of group A2 and B2,and the differences were statistically significant（ P〈0. 05）. The IL-6 level of group A1 was higher than that of group B1,and the difference was statistically significant（ P〈0. 05）. Conclusion： IL-6 and IL-17 in BALF can be used as useful indexes to reflect the severity of severe pneumonia in children with acute pneumonia.