艾灸益气活血法对D-半乳糖致衰老大鼠免疫功能的影响

Effect of Enriching Qi and Activating Blood Circulation of Moxibustion on the Immune Function of D-galactose Induced Aging Rats

目的研究艾灸对D-半乳糖所致衰老大鼠免疫功能的影响,探讨艾灸抗衰老的机制。方法雄性SD大鼠,采用D-半乳糖皮下注射制备亚急性衰老大鼠模型,随机分为模型组、艾灸组、吡拉西坦组,另设空白组作为对照,每组10只。30 d后,测定胸腺、脾脏指数,MTT法检测淋巴细胞转化刺激指数,ELISA法测定血清转化生长因子-β(TGF-β)、干扰素-γ(IFN-γ)含量。结果与空白对照组比较,模型组大鼠胸腺、脾脏指数明显降低,T淋巴细胞和B淋巴细胞增殖活性下降,TGF-β含量升高,IFN-γ含量下降;与模型组相比,艾灸组胸腺、脾脏指数明显升高,T淋巴细胞和B淋巴细胞增殖活性上调,TGF-β含量下降而IFN-γ含量升高,表明艾灸可明显提高衰老大鼠T、B淋巴细胞增殖能力,降低TGF-β含量,升高IFN-γ含量。结论艾灸益气活血法可通过增强衰老大鼠免疫功能,达到延缓衰老的目的。

Objective To study the effect of moxibustion on the immune function of D-galactose induced aging rats,and to explore the mechanism of moxibustion in anti-aging. Methods The sub-acute aging rat models were prepared by subcutaneous injection of D-galactose in male SD rats. The groups were randomly divided into Model group,Moxibustion group and Piracetam group with 10 rats in each group. Besides,the blank group was adopted as the control group with 10 rats in it. After 30 days,the thymus and spleen index were measured. The lymphocyte transformation stimulation index was detected by MTT,and the content of transformation growth factor-β（TGF-β） and interferon-（IFN-γ） in serum was measured by ELISA. Results Compared with the blank control group,the thymus and spleen index decreased significantly in the model group; the proliferation activity of T and B lymphocytes decreased;the content of TGF-β increased; the content of IFN-γ decreased. Compared with the model group,the thymus and spleen index of the moxibustion group increased significantly,and the proliferation activity of T lymphatic cells and B lymphocytes were increased. The decrease of TGF-β content and the increase of IFN-γ content showed that moxibustion could significantly increase the proliferation ability of T and B lymphocytes,reduce the content of TGF-β and increase the content of IFN-γ in aging rats. Conclusion Enriching Qi and activating blood circulation of moxibustion can enhance the immune function of aging rats and achieve the purpose of delaying senility.