免疫亲和层析净化高效液相色谱法和胶体金快速定量法测定小麦中呕吐毒素含量的比较

Comparison of determination of deoxynivalenol content in wheat by immunoaffinity chromatography(HPLC)and colloidal gold rapid quantification method

采用免疫亲和层析净化高效液相色谱法和胶体金快速定量法测定小麦中呕吐毒素含量。结果表明:用这两种方法测定添加不同浓度呕吐毒素的小麦,测定结果的相对标准偏差分别为1.59%~3.08%和7.28%~11.50%,且两种方法测定呕吐毒素呈阳性的小麦样品的测定结果相对偏差小,相关系数为0.995,相关性好。这两种方法各有优缺点,免疫亲和层析净化高效液相色谱法精密度好,能够对呕吐毒素进行精准定量,但试验步骤较为复杂,耗时长;胶体金快速定量法检测简便快速,能用于大量样品的快速筛查,预期在粮食中真菌毒素检测方面具有很好的应用前景。

The content of deoxynivalenol（DON）in wheat was determined by immunoaffinity chromatography（HPLC）and colloidal gold rapid quantification method.The results showed that the relative standard deviation（RSD）of wheat determined by the two methods were 1.59%-3.08% and 7.28%-11.50%,respectively.The results showed that the relative deviation of wheat samples with positive deoxynivalenol between the two methods was very small,the correlation coefficient is 0.995,and the correlation was good.The methods could accurately quantify the DON by immunoaffinity chromatography purification HPLC,but the experimental steps were complicated and time-consuming.The colloidal gold rapid quantification method could be used for rapid screening of a large number of samples,and it had a good application prospect in the detection of mycotoxin in grain.