番石榴叶抑制db/db小鼠肾脏TGF-β1蛋白表达研究

Guava leaf extracts decrease renal tissue TGF-β1 Immunohistochemistry expression in db/db mice

目的:观察番石榴叶提取物对自发2型糖尿病db/db小鼠肾脏HE染色及TGF-β1免疫组化的影响,探讨其肾脏保护的机制。方法:10周龄雄性肥胖db/db小鼠16只,适应性喂养2周后,随机分为模型组和番石榴叶提取物组,所有动物给予高脂饲料喂养,番石榴叶提物组按照0.11g/kg灌胃(相当于生药材2g/kg),模型组给予同体积蒸馏水灌胃,疗程为12周。每4周观察体重、血糖变化,试验结束后,HE染色观察肾脏病理变化,免疫组化法观察TGF-β1蛋白表达。结果:与对照组相比,经过12周的治疗后,番石榴叶提取物显著降低db/db小鼠体重和血糖。HE染色结果显示,番石榴叶提取物可以显著改善db/db小鼠肾小球系膜细胞增生、球旁器增生,颗粒样变性。免疫组化结果提示,番石榴叶提取物可以显著下调db/db小鼠肾脏TGF-β1蛋白表达(P<0.05)。结论:番石榴叶提取物通过抑制肾脏组织TGF-β1免疫组化蛋白表达,改善db/db小鼠糖尿病肾病肾脏纤维化。

Objective：To observe treatment of Guava leaf extracts on renal tissue TGF-β1 protein expression in db/db mice.Methods：16 male obese db/db mice,10 weeks old,were randomly divided into control group and Guava leaf water extracts（0.11 g/kg）after being fed for 2 weeks.All mice received high fat diet and 12-week treatment.Body weight and glucose were tested every week.HE staining according to the manufacturers＇ instructions.Renal tissue TGF-β1 protein was tested by immunohistochemistry,The relative levels of each target protein to controlβ-actin were determined by densitometry analysis using the Image-Pro Plus.Results：GL extracts significantly lowered the values of body weight and plasma glucose,reduced the proliferation of glomerular mesangial cells,proliferation of peripheral organs,and granular degeneration.Furthermore,treatment with GL extracts decreased renal tissue TGF-β1 protein expression by immunohistochemistry.Conclusion：GL extracts may improve fibrosis by decrease TGF-β1 signaling pathway in renal tissue.