抗肿瘤溶栓嵌合蛋白制备工艺研究

Studies on Preparation of Chimeric Proteins Possessing Anti-tumor and Thrombolytic

对抗肿瘤溶栓嵌合蛋白在重组大肠杆菌中高效表达作了研究.以装液量、接菌量和初始pH值三因素设计正交试验,确定其最佳培养条件.结果表明:抗肿瘤溶栓嵌合蛋白制备工艺的最佳培养条件是装液量100 m L、接菌量2%、初始pH值7.5.在优化后的培养条件下,1 L发酵液可以得到0.987 mg蛋白,比优化前蛋白量提高了一倍,较高的表达量为进一步研究该蛋白的纯化和性质提供了基础.

The high express of chimeric proteins possessing anti-tumor and thrombolytic in E.coli was studied. The optimum cultivating conditions were determined through orthogonal test of culture volume,seed dose and medium original pH value. Results showed that the optimum cultivating conditions for preparation of chimeric proteins possessing anti-tumor and thrombolytic were summarized as follows： culture volume is 100 m L,seed dose is 2%,medium original pH value is 7. 5. Under the optimum cultivating conditions,1 L fermented liquid can get 0. 987 mg protein,which was 1-fold of the initial protein porduction. The relative high protein porduction was helpful for further study on the purification and characterization of the protein.