银离子注入氧化钛涂层抗金黄色葡萄球菌的体外效果评价

In vitro evaluation of silver ion implantation into titanium dioxide coating against Staphylococcus aureus

背景:制备缓释、长期释放的银离子抗菌涂层,仍然是材料表面改性抗菌的重要课题之一。目的:评价银离子注入氧化钛涂层后体外抗金黄色葡萄球菌的特点及其效果。方法:对照组为纯钛组,实验组分为3组,为氧化钛涂层组、氧化钛涂层+低银离子组(30 min)和氧化钛涂层+高银离子组(60 min);扫描电镜观察处理后材料的特征,运用细菌滴入法、抑菌环实验和菌液生长曲线法来评价抗菌效果。结果与结论:通过银离子注入技术,可以将银离子加载至氧化钛涂层表面;体外实验证实银离子注入后的材料具有明显的抗菌性,且随着注入时间的延长,材料表面银离子的数量增加,抗菌性明显提高;银离子注入技术用于制备无机抗菌材料经过体外实验评价具有抗菌能力强、抗菌持久等特点。

BACKGROUND： It is a challenge to seek for ideal anti-bacterial surface modification by which silver ions can have sustained and durable release. OBJECTIVE： To investigate the in vitro characteristics and effect of titanium dioxide coating with silver ion injection against Staphylococcus aureus . METHODS： There were four groups in the study： control group （pure titanium）, titanium oxide coating group, low silver ion group （titanium oxide coating＋30 minutes injection of silver ions）, and high silver ion group （titanium oxide coating＋60 minutes injection of silver ions）. Characteristics of the implanted material in each group were observed using scanning electron microscope. Antibacterial efficacy for Staphylococcus aureus was assessed through bacterial instillation method, antibacterial ring test, and microbial growth curve. RESULTS AND CONCLUSION： Silver ions could be loaded onto the surface of the titanium dioxide coating through the silver ion implantation. Moreover, the number of silver ions on the coating surface and the antibacterial ability of the modified coating were both increased with the extension of injection time. Therefore, the silver ion implantation technology is beneficial to prepare inorganic antibacterial materials with strong and long-lasting antibacterial ability.