中药复方癌宁对人胃腺癌细胞凋亡基因表达的影响

Effect of Traditional Chinese Medicine Compounds Aining on the Expression of Apoptosis Inducing Genes of Human Gastric Cancer Cell

目的:探索癌宁对体外人胃癌细胞基因表达的影响。方法:采用流式细胞术,检测了中药复方癌宁及顺铂诱导胃癌细胞凋亡基因的表达。结果:顺铂、癌宁组与空白对照比较,bcl-2、c-myc基因表达产物含量显著降低,p^(53)、bax基因表达产物含量显著增高(P<0.01),bax/bcl-2比值显著增高。结论:癌宁对人胃癌细胞的诱导凋亡作用与其对癌基因、抑癌基因表达的改变有关。

Objective: To investigate the effects of Aining on the genes of the human gastric cancer cells. Methods: Flow cytometry( FMC) was adopted to detect the gene expression of the gastric cancer cell in the Aining group, the cisplatin(DDP)group and the blank group. Results: Compared with the blank group, the expression of c-myc and bcl-2 genes was low, while that of the p53,bax genes was high(P< 0.01) ,and the ratio of bax/bcl-2 significantly increased in both the Aining group and the cisplatin(DDP)group. Conclusions: The mechanism of inducing the human gastric cancer cells apoptosis by Aining has some relationship with the changes of the genes.