双孢菇多糖的提取及抗氧化活性研究

The Extraction and Antioxidant Activity of Polysaccharides in Agaricus bisporus

本文采用单因素试验和正交试验对双孢菇多糖的提取工艺进行优化,并对双孢菇多糖的抗氧化活性进行分析。试验结果表明,双孢菇多糖提取的最优参数为:提取温度80℃,提取时间120min,液料比为40:1(mL:g,下同)。在此条件下,双孢菇多糖提取率为1.53%。然后对双孢菇多糖的还原力,Fe^(2+)的螯合性,对DPPH自由基、羟基自由基和超氧阴离子自由基的清除能力进行了测定,结果发现双孢菇多糖具有良好的抗氧化活性,应用前景较广阔。

The main single factor experiments and orthogonal experiments were used to study extraction processing of polysaccharides from Agaricus bisporus, and the antioxidation activity of polysaccharides was analyzed. Experimental results showed that optimal processing of polysaccharides from Agaricus bisporus was that the temperature was 80~C, the extraction time was 120rain, the rate of liquid to material was 40：l（mL：g）. Under the condition of the optimal processing, the extraction rate of polysaccharides from Agaricus bispoms could be reached 1.53%. In the paper, the reducing capacity, the chelating ability of Fe2＋, the scavenging capacity of DPPH radical, scavenging hydroxyl radical and superoxide anion radical were determined. The results certified that polysaccharides from Agaricus bispoms has good antioxidant capacity and has broad