PTEN基因对急性淋巴细胞白血病细胞增殖、侵袭的影响

Effect of PTEN on the proliferation and invasion of acute lymphoblastic leukemia

目的:观察第10号染色体同源缺失性磷酸酶-张力蛋白同源的基因(PTEN)对急性淋巴细胞白血病细胞增殖、侵袭的影响。方法:在人急性淋巴细胞白血病细胞CEM-C1中转染PTEN过表达载体(p BPPTEN),同时转染对照载体(p BP),以没有转染的细胞作为对照,RT-PCR和Western blot检测转染后细胞中PTEN的水平;MTT和集落形成试验检测细胞增殖能力;Transwell小室检测细胞侵袭和迁移能力;Western blot检测PTEN、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)、p38丝裂原活化蛋白激酶(p38MAPK)、磷酸化的p38MAPK(p-p38MAPK)的水平。结果:转染对照载体的CEM-C1细胞中PTEN水平、细胞光密度(OD)值、克隆形成数目、侵袭数目、迁移数目及PTEN、MMP-2、MMP-9、p38MAPK、pp38MAPK水平与未转染细胞相比没有明显变化(P>0.05)。转染PTEN过表达载体后的CEM-C1细胞中PTEN水平和p-p38MAPK水平升高,细胞OD值、克隆形成数目、侵袭数目、迁移数目和MMP-2、MMP-9水平降低,与未转染细胞相比差异具有统计学意义(P<0.05)。结论:PTEN抑制急性淋巴细胞白血病细胞增殖、侵袭及迁移,促进p38MAPK信号通路激活。

Objective： To investigate the effect of PTEN on the proliferation and invasion of acute lymphoblastic leukemia cells. Methods： In human acute lymphoblastic leukemia cells,CEM-C1 was transfected with PTEN overexpression vector（ p BP-PTEN） and transfected into the control vector（ p BP）,the cells without transfection were used as controls. The levels of PTEN in transfected cells were detected by RT-PCR and Western blot,MTT and colony forming assays were used to detect cell proliferation,the cell invasion and migration ability was detected by Transwell.PTEN,MMP-2,MMP-9,p38MAPK,p-p38MAPK protein levels were detected by Western blot. Results： PTEN levels,OD value,colony forming number,invasion number,migration number and PTEN,MMP-2,MMP-9,p38MAPK,p-p38MAPK levels in CEM-C1 cells transfected with the control vector had no obvious changes compared with the non transfected cells（ P〉0. 05）. After the transfection of PTEN increased expression of PTEN and p-p38MAPK level vector in CEM-C1 cells,cell clone formation,invasion number,OD value,migration number and MMP-2,MMP-9 level reduced compared with non transfected cells,the difference was statistically significant（ P〈0. 05）. Conclusion： PTEN inhibits the proliferation,invasion and amigration of acute lymphoblastic leukemia and promotes the activation of p38MAPK signaling pathway.