电针对具有胃动力障碍的抑郁模型大鼠行为学及胃排空的影响

Influence of Electroacupuncture on Behavior and Gastric Emptying in Depression Model Rats with Gastric Motility Disorder

目的观察电针对具有胃动力障碍的抑郁模型大鼠行为学及胃排空的影响。方法 SD大鼠60只,随机选取16只为对照组,余44只采用慢性轻度不可预见性应激(chronic unpredictable mild stress,CUMS)配合孤养的方法制备抑郁模型,用旷场实验确定抑郁模型复制是否成功、胃电图确定抑郁模型大鼠是否具有胃动力障碍(造模组剔除12只),再从对照组和造模组各随机选取8只进行胃排空检测,验证抑郁模型具有胃动力障碍,造模组剩余的24只大鼠随机分为模型组、针刺组、百忧解组,每组8只。对照组、模型组束缚处理,生理盐水灌胃;针刺组束缚处理,生理盐水灌胃,针刺足三里、太冲、肝俞、胃俞、中脘穴,中脘穴只针刺、不接电,余穴接电针;百忧解组束缚处理,百忧解灌胃。干预结束后,处死所有大鼠检测各组大鼠胃排空率;取血清检测血浆P物质(substance P,SP)和降钙素基因相关肽(calcitonin gene related peptide,CGRP);取脑组织检测多巴胺(dopamine,DA)。结果 (1)电针能增加模型大鼠旷场实验水平方向总位移和垂直运动次数(P<0.01),同时增加胃排空率(P<0.05);(2)电针能促进模型大鼠脑组织DA表达(P<0.05)和血清SP表达(P<0.01),抑制血清CGRP表达(P<0.01)。结论电针可同时改善抑郁模型大鼠抑郁和胃动力障碍症状,其作用机制可能是通过促进DA和SP表达、抑制CGRP表达实现的。

Objective To observe the influence of electroacupuncture on behavior and gastric emptying in depression model rats with gastric motility disorder. Methods The 16 rats selected from 60 SD rats were assigned into the control group, The depression models of remaining 44 rats were prepared under the conditions of chronic unforeseeable mild stress （CUMS） and separation. The rat model of depression was determined by open field test. The gastric motility disorders of depression model rats were detected by electrogastrogram （12 rats were removed from the rat model）. Then 8 rats were selected from each the control group and the model group, and the gastric emptying was detected. The remaining 24 models were randomly divided into acupuncture group, fluoxetine group and model group were given bound-bound treatment and acupunctureed at Zusanli （ST36）, Taichong （LR3）, Ganshu （BL18）, and model group, 8 rats in gavaged with normal saline each group. The control group The acupuncture group were Weishu （BL21）, Zhongwan （RN12） acupoints. Zhongwan acupoint was given needling and with no electricity, and other acupoints were received electroacupuncture. Fluoxetine group was administrated with fluoxetine. After the intervention, all rats were killed to detect the gastric emptying rate, substance P （SP） and calcitonin gene related peptide （CGRP） in serum were detected, and dopamine （DA） was detected in the brain tissue. Results （1） electroacupuncture could increase the level of total displacement and vertical movement （P〈0.01）, and increase the rate of gastric emptying （P〈0.05）. （2） Electroacupuncture could promote the expression of DA （P〈0.05） and the expression of SP （P〈0.01） in the brain tissue of rats and inhibit the expression of CGRP in serum （P〈0.01）. Conclusion Electroacupuncture can improve the symptoms of depression and gastric motility in rats with depression. Its mechanism may be promoting the expression of DA and SP, and inhibiting the expression of CGRP.