妊娠滋养细胞疾病组织中HIF-1α、VEGF的表达及临床意义

The Expression and Clinical Significance of HIF-1α and VEGF in the Tissues of Gestational Trophoblastic Disease

目的:检测缺氧诱导因子1α(HIF-1α)和血管内皮生长因子(VEGF)在正常早孕绒毛、葡萄胎和妊娠滋养细胞肿瘤(GTN)组织中的表达情况,并探究其临床意义。方法:收集正常早孕绒毛组织20例(正常早孕绒毛组)、葡萄胎组织35例(葡萄胎组)和GTN组织28例[包括侵蚀性葡萄胎组织14例(侵蚀性葡萄胎组),绒毛膜癌组织14例(绒毛膜癌组)]。采用免疫组化法检测各组织样本中HIF-1α和VEGF的蛋白表达情况。分析GTN患者不同年龄及FIGO分期组织中HIF-1α和VEGF表达的情况。分析GTN组织样本中HIF-1α和VEGF表达的关系。结果:正常早孕绒毛组与葡萄胎组HIF-1α和VEGF蛋白表达的阳性率比较,差异无统计学意义(均P>0.05);正常早孕绒毛组HIF-1α和VEGF蛋白表达的阳性率低于侵蚀性葡萄胎组和绒毛膜癌组,差异有统计学意义(均P<0.05);侵蚀性葡萄胎组和绒毛膜癌组HIF-1α和VEGF蛋白表达的阳性率比较,差异无统计学意义(均P>0.05)。GTN患者FIGOⅢ+Ⅳ期组HIF-1α和VEGF蛋白表达的阳性率高于Ⅰ+Ⅱ期组,差异有统计学意义(均P<0.05);而不同年龄GTN患者HIF-1α和VEGF蛋白表达的阳性率比较,差异无统计学意义(均P>0.05)。HIF-1α与VEGF在GTN组织中的表达呈正相关(rs=0.995,P=0.000)。结论:HIF-1α及VEGF可能在GTN发生和恶性进展过程中发挥协同作用。

Objective：To detect the expression of HIF-1α and VEGF in the tissues of normal pregnancy villi,hydatidiform moles and gestational trophoblastic neoplasm（GTN）, and to explore the clinical significance. Methods：20 cases of normal pregnancy villi（normal pregnancy villi group）, 35 cases of hydatidiform moles（hydatidiform moles group）, 28 cases of GTN tissues [concluding 14 cases of invasive moles（invasive moles group） and 14 cases of choriocarcinoma（choriocarcinoma group）] were collected. The protein expression of HIF-1α and VEGF in the tissues were detected by the immunohistochemistry assay. The expression of HIF-1α and VEGF in different age, FIGO stage of GTN patients were analyzed. The correlation between HIF-1α and VEGF expression in GTN tissue samples was analyzed. Results：There was no significant difference in the positive rate of HIF-1α and VEGF protein between the normal early pregnancy villi group and the hydatidiform mole group（ P〉0.05）.The positive rates of HIF-1α and VEGF in the normal pregnancy villi group and hydatidiform mole group were much lower than those in the invasive hydatidiform mole group and choriocarcinoma group（P〈0.05）. Moreover, there was no difference in the positive rates of HIF-1α and VEGF between the invasive moles group and choriocarcinoma group（P〉0.05）. In the GTN tissues,the expression of HIF-1α and VEGF were correlated with the FIGO stages（P〈0.05）, but were not correlated with the age（P〉0.05）. The expression of HIF-1α and VEGF were positively related in the GTN tissues（rs=0.995, P=0.000）. Conclusions：HIF-1α and VEGF may play a synergistic role in the occurrence and malignant progression of GTN.