心房钠尿肽在基因敲除小鼠黑色素瘤肺转移中的作用

Effect of atrial natriuretic peptide(ANP) on pulmonary melanoma metastasis in ANP knockout mice and C57BL/6J mice

目的探讨心房钠尿肽(atrial natriuretic peptide,ANP)对黑色素瘤肺转移的作用。方法将体外培养的黑色素瘤B16F10细胞株通过尾静脉注射到心房钠尿肽敲除小鼠(ANP-/-小鼠)和C57BL/6J小鼠的体内,建立黑色素瘤肺转移模型,模型建立后第21天计数转移到肺表面的结节数目,并把肺组织包埋固定、切片、HE染色后,确定肺转移结节并计数。结果 ANP敲除小鼠肺表面转移的黑色素瘤结节数明显比C57BL/6J小鼠少,差异有显著性;ANP敲除小鼠肺微转移结节数目也明显比C57BL/6J小鼠少,差异有显著性。结论 ANP敲除显著抑制了黑色素瘤的肺转移。

Objective To investigate the role of atrial natriuretic peptide （ANP） in lung metastasis of melanoma.Methods Melanoma B16F10 cells were intravenously injected into ANP knockout mice and C57BL/ 6J mice. After threeweeks, the mice were sacrificed, and the lungs were removed, embedded, and examined by pathology using HE staining.The numbers of metastatic foci on the lung surface and micrometastatic foci in the lung tissues were counted. Results TheANP knockout mice displayed fewer metastatic foci on the lung surface and less micrometastatic foci in the lung tissues ofANP knockout mice than in the C57BL/ 6J mice. Conclusions ANP deletion significantly suppresses the metastasis ofmelanoma in the lung of mice.