鹌鹑不同采血方法对比与评价

Comparison and evaluation of three different blood sampling methods in quails

目的评价鹌鹑三种采血方法的特点。方法分别对三组鹌鹑翼根、左颈部、右颈部静脉采血,比较三部位采血的难度、血清质量、采后出血量、采血后状态等,评价三种采血方式。结果所有鹌鹑存活良好,右颈静脉采血难度相对小、血清质量佳、采血后鹌鹑出血量小、状态良好,伤口易恢复。左颈静脉采血难度一般,血清质量佳、采血后鹌鹑出血量较大、采后状态一般;鹌鹑翼根静脉采血难度相对较大,不宜大量采血、采血后鹌鹑出血量较大。结论鹌鹑大量采血时可选用左右颈静脉采血法,少量或微量采血时三种方法均可采用。

Objective To evaluate the characteristics of three blood sampling method in quails. Methods 300healthy 5-week old male quails were randomly divided into 3 groups： blood samples were taken from the wing root vein, theleft jugular vein, and the right jugular vein, respectively, 100 quails in each group. The advantages and disadvantages ofthe three methods of blood sampling were compared, including the difficulty of blood collecting, the quality of blood serumobtained, the amount of bleeding after venepuncture, and the general condition of the quails after blood collection. ResultsAll quails survived well. The right jugular vein was relatively small, and the quality of obtained serum was good, thebleeding amount after blood collection was small, the wound was easy to recover, and the general condition of the quailswas good. The left jugular vein method was a little bit more difficult to collect blood, the quality of the obtained serum wasgood, the bleeding amount of quails after blood collection was larger, and the general state post puncture was rather good.The blood collection from the wing root vein was relatively difficult, with more massive bleeding after the vein puncture,therefore, is not suitable for large amount blood collection. Conclusions Blood samples in a large amount can be collectedfrom both the right or left jugular veins. Small quantity of blood samples can be collected by either one of the three method.