三七总皂苷微乳对帕金森模型小鼠行为学及纹状体多巴胺含量的影响

Effects of Panax Notoginseng Saponins microemulsion on behavior and striatal dopamine content in Parkinson's model rats

[目的]研究三七总皂苷微乳及溶液对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森模型小鼠的治疗作用,为后期药代动力学及PK-PD研究奠定基础。[方法]首先成功复制MPTP所致帕金森病的小鼠模型,连续5 d腹腔注射MPTP(30 mg/kg)。于造模起第3天给予三七总皂苷微乳及溶液(100 mg/kg)预处理MPTP所致的帕金森模型小鼠。于最后1次给药后进行行为学指标的测试,行为学实验结束后第2天各组小鼠断头取脑,用高效液相色谱-荧光检测器测定多巴胺(DA)含量。[结果]在MPTP所致的帕金森模型小鼠中,其行为学障碍明显;三七总皂苷微乳及溶液治疗组,行为学障碍略有改善。与正常组比较,模型组DA含量显著降低(P<0.01),PNS微乳及溶液给药组DA含量显著升高(P<0.01),且两组间有统计学差异(P<0.01)。[结论]三七总皂苷微乳对帕金森模型小鼠有明显的治疗作用,且三七总皂苷微乳组的效果优于溶液组。

[Objective] To study the effective onset time of panax notoginseng saponin microemulsion on 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine（MPTP）-induced Parkinson＇s mouse model, which is lay the fundation of the pharmacokinetics and PK-PD study. [Methods] First, MPTP-induced mouse model of Parkinson＇s disease was injected intraperitoneally with MPTP（30 mg/kg） for 5 days. On the third day after the model was given, Panax notoginseng microemulsion and solution（100 mg/kg） were given MPTP-induced Parkinson＇s model rats. After the last day of administration behavioral tests index, the next day after the behavioral experiments in each group decapitation brains were removed by HPLC-Determination of Dopamine in a fluorescence detector. [Results] Mice MPTP induced Parkinson in which significant behavioral disorders; Panax notoginseng saponins microemulsion and solution treatment group, a slight improvement in behavioral disorders. Compared with the normal group, the dopamine content in the model group was significantly lower（P〈0.01）, the dopamine content in the PNS microemulsion and solution administration group was significantly higher（P〈0.01）, and there was a significant difference between the two groups（P〈0.01）. [Conclusions] Panax notoginseng saponins microemulsion on the Parkinson model mice have a significant therapeutic effect, he effect of Panax notoginseng microemulsion group was better than that of solution group.