多巴胺转运体减少对于味觉偏好转化的影响

Dopamine Transportor Hypoexpression Influences the Taste-to-nutrition Suitability

目的:通过建立小鼠味觉转化模型,研究多巴胺转运体(dopamine transporter,DAT)对这一行为的作用。方法:使用20只DATIRES CRE+/-的杂合小鼠和20只DATIREScre-/-的对照小鼠进行一系列味觉相关的行为学测试,通过Western Blot检测小鼠体内DAT蛋白在不同脑区的表达情况。结果:DATIREScre杂合组和对照组相比,其先天性味觉选择以及营养感受与分辨没有异常,但是在味觉感受转化时,尤其是在厌恶性味觉向偏好性味觉的转化中出现异常。结论:消化后营养感受需要多巴胺系统参与,多巴胺转运体表达降低会干扰这一重要过程。这可能是临床上注意力缺失过动症儿童挑食症状的潜在机制。

Object： To analyze the influence of DAT（dopamine transporter） hypoexpression in the taste-to-nutrition suitability. Method： 20 DAT^IREScreheterozygous and littermates wild type controls were tested with series of taste behavior assays, also western blot was applied to measure the DAT expression in different brain regions. Results： Compared with the littermate control, heterozygous mice showed normal taste and nutrition sensation and preference; however, they expressed abnormalities when the taste and post-ingestive nutrition were mismatched. Conclusion： Dopamine information is necessary for coding nutrition valances. DAT hypoexpression interferes the nutritional sensing. This may be the potential mechanism of anorexia in the attention deficit hyperactivity disorder.