利用全局转录调控工程提高菌株耐受性研究进展

Global Transcription Machinery Engineering to Improve Strain Tolerance

微生物在不同环境胁迫下的耐受性对于生物燃料和生物化学品的高效生产有重要意义.目前广泛应用于提高微生物菌株耐受性的方法包括过表达的自身或外源与耐受性相关的基因、适应性进化、人工诱变或基因组改组等.近年来,人们试图通过全局转录工程调控蛋白直接或间接操纵转录调控网络,以提高微生物胁迫耐受性.本文就利用全局转录调控工程提高菌株耐受性的研究进展包括对提高乙醇耐受性、耐酸性、耐有机溶剂、氧化应激耐受性以及耐糖性进行综述,阐明利用全局转录调控工程提高微生物耐受性所具有的广阔前景.

Microbial tolerance under different environmental stresses is important for the efficient production of biofuels and biochemicals.Methods widely used for strain tolerance improvement include overexpression of well-characterized native or exogenous resistantstructural genes,long-term adaptive evolution,artificial mutagenesisor genome shuffling combined with selection,and so on.In recent years,attempts have been made for direct or indirect manipulation of the transcriptional regulation network by engineering transcriptional regulatory proteins to enhance the microbial stress tolerance of industrial relevance.This paper summarized the advancement of the research to improve strain tolerance through global transcription machinery engineering,including studies to improve ethanol tolerance,acid resistance,resistance to organic solvents,oxidative stress tolerance and resistant to sugar.It showed that this methodology has great prospects for improving microbial stress tolerance.