对叶百部药材中四种主要生物碱的分布规律

Distribution of Four Main Alkaloids in the Root of Stemona tuberosa

目的:了解百部药材中主要生物碱的含量和分布规律,为百部药材的质量控制提供依据。方法:采用反向HPLC-ELSD方法对百部药材中4种主要生物碱金刚大碱、百部新碱、新对叶百部碱和对叶百部碱的含量就行了测定,以0.1%三乙胺-甲醇(43∶57)为流动相,Agilent C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,流速1m L/min;ELSD漂移管温度为80℃,氮气流速为2.1 m L/min,增益为8。结果:83批百部药材含金刚大碱、百部新碱、新对叶百部碱和对叶百部碱的药材分别有27、48、22和48批,其平均含量分别为1.98、4.75、3.73和5.34 mg/g;含有1~4种生物碱的药材分别有38、28、17和0批次,其中对叶百部碱与其余三种生物碱中任意一种同时出现的概率较高;购于安徽亳州和广州清平药市的药材不含新对叶百部碱。结论:本文建立的含量测定方法简便、准确、可靠;含量测定结果说明4种生物碱在药材中的分布具有趋单性,仅含一种生物碱的药材占45.8%,含2种及以上生物碱的药材占54.2%。本文研究结果对于对照品短缺情况下,对购买百部药材化学成分的预测具有一定的指导意义。

Objective： To understand the distribution of the alkaloids in Baibu（the root of Stemona tuberosa）,the contents of the main alkaloids were determined. Methods： A reverse HPLC-ELSD method was established for the determination of croomine,stemoninine,neotuberostemonine and tuberostemonine in Baibu. They were separated on an Agilent Extend C18 column（4. 6 mm × 250 mm,5μm） with a mobile phase of 0. 1% triethylamine-methanol（43∶ 57）,a column temperature of 30℃ and a flow rate of1 ml/min. ELSD was set to 80℃ for drift tube,2. 1 ml/min for N2 and 8 for gain. Results： Among the 83 batches of Baibu,the batches containing croomine,stemoninine,neotuberostemonine and tuberostemonine were 27,48,22 and 48,respectively,with an corresponding average content of 1. 98,4. 75,3. 73 and 5. 34 mg/g. There were 38 batches containing one alkaloid,28 batches containing 2 ones,17 batches containing 3 ones and no batches containing 4 ones. Tuberostemonine are readily associated with the other alkaloids. Baibu purchased from Bozhou（Anhui） and Qingping（Guangzhou） didn＇t contain neotuberostemonine. Conclusion： The established HPLC-ELSD method was simple,accurate,and reliable. The distribution of the alkaloids tended to be single because 45. 8% batches of Baibu contained only1 alkaloid. This study has some guiding significance for the prediction of the chemical constituents of Baibu under the shortage of reference substance.