胚胎干细胞分泌因子增强AML-12细胞抗凋亡能力

Embryonic stem cell secreted cytokines in the enhancement of AML-12 cells anti-apoptotic ability

目的:研究胚胎干细胞分泌因子是否能增强AML-12细胞在恶劣条件培养下的抗凋亡能力。方法:分别用胚胎干细胞完全培养基(CON-M)和胚胎干细胞上清液(ESC-M)培养AML-12细胞,并置于恶劣细胞培养环境中培养,MTT法比较各组AML-12细胞活力;Seahorse细胞代谢分析仪比较各组AML-12细胞氧消耗率以及细胞外酸化率等呼吸功能代谢情况。结果:MTT结果显示在恶劣细胞培养环境中,于ESC-M中培养的AML-12细胞拥有更强的抗凋亡能力。Seahorse细胞代谢分析显示ESC-M培养能加强AML-12细胞糖酵解潜力、最大氧化呼吸能力以及氧化呼吸潜力。结论:胚胎干细胞分泌因子能够增强AML-12细胞在恶劣培养条件下的抗凋亡能力。

Objective： To investigate whether the embryonic stem cell secreted cytokines can enhance AML-12 cell anti-apoptotic ability in extreme cell culture conditions. Methods： AML-12 cells were respectively treated with embryonic stem cells complete media（CON-M） or embryonic stem cells conditioned media（ESCM） and cultured in extreme cell culture conditions. Cells viability was detected by MTT assay. Mitochondrial energy metabolism including oxygen consumption rate and extracellular acidification rate were measured using a Seahorse metabolic flux analyzer. Results： MTT assay indicated that AML-12 cells treated with ESC-M had stronger anti-apoptotic abilities in extreme cell culture conditions. Results from the Seahorse metabolic flux analyzer showed that ESC-M could increase AML-12 cells maximal respiration capacity, spare respiration capacity as well as glycolytic capacity. Conclusion： The embryonic stem cells secreted cytokines enhance AML-12 cells anti-apoptotic abilities in extreme cell culture conditions.