利用常压室温等离子体诱变技术选育高产黄酮的桑黄菌株

Rational Seletion of Phellinus baumii Mutants for Improved Intracellular Flavonoids Biosynthesis by Atmospheric Room Temperature Plasma Mutagenesis

为提高桑黄(Phellinus baumii)黄酮生产能力,利用常压室温等离子体(atmospheric room temperature plasma,ARTP)技术诱变桑黄菌株SH1(出发菌株)的原生质体,经过筛选获得了3株高产胞内黄酮的优势菌株A67、A130、A157,与出发菌株相比,菌株A67、A130、A157液体发酵胞内总黄酮产量分别提高了86.7%、20.0%和60.0%。拮抗实验和RAPD分析显示3株优势菌株与出发菌株间存在遗传差异。TEAC(Trolox equivalent antioxidant capacity)和FRAP(ferric reducing antioxidant power)实验结果表明,A67菌丝体醇提物比出发菌株具有更好的体外抗氧化活性。本研究为桑黄属菌株的选育提供了高效可行的诱变方法,并为桑黄黄酮相关产品的开发和应用提供了优良的新菌株。

To increase total flavonoids in Phellinus baumii mycelia, protoplasts from the wild strain were treated by the room temperature plasma （ARTP） mutagenesis. Three resultant strains, A67, A130, and A157, showed both high flavonoids yields and genetic stability after five consecutive subcultures. Compared to the control strain SH1, total flavonoids content of A67, A130, A157 strains increased by 86.7%, 20.0% and 60.0%, respectively. The antagonistic experiment and the RAPD analysis indicated genetic differences between the three selected mutants and the control strain. Compared to the control strain, the ethanol extracts of A67 mycelia exhibited stronger antioxidant activities in vitro, contained higher Trolox equivalent antioxidant capacity （TEAC） and ferric reducing ability of plasma （FRAP） values. The results of this study suggested that ARTP-induction is an efficient and practical method for Phellinus strain improvement and thus provided a basis for the development and application of P. baumii flavonoids related products.