竹节蓼药材的HPLC指纹图谱建立及聚类分析

Establishment of HPLC Fingerprint of Homalocladium platycladum and Cluster Analysis

目的建立竹节蓼药材的高效液相色谱(HPLC)指纹图谱,并进行聚类分析。方法:采用HPLC法。色谱柱为Ultimate C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为0.5 mL/min,检测波长为360 nm,柱温为28℃,进样量为10μL。以槲皮苷为参照,绘制13批药材样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2012版)进行相似度评价,确定共有峰,并对其进行聚类分析。结果:13批药材样品的HPLC图谱有12个共有峰,相似度均大于0.90;经验证,13批药材样品的HPLC图谱与对照指纹图谱具有较好的一致性。13批药材样品可聚为两大类,S4、S6、S10聚为一类,其余聚为一类。结论:该研究所建指纹图谱和聚类分析结果可为竹节蓼药材的质量评价及进一步开发利用提供参考。

OBJECTIVE：To establish HPLC fingerprint of Homalocladium platycladum,and to carry on the cluster analysis.METHODS：HPLC method was adopted. The determination was performed on Ultimate C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid（gradient elution）at the flow rate of 0.5 mL/min. The detection wavelength was set at 360 nm,and column temperature was 28 ℃. The sample size was 10 μL. Using quercetin as reference substance,HPLC chromatograms of13 batches of samples were determined. The similarity evaluation was carried out by using Similarity Evaluation System for TCM Chromatographic Fingerprint（2012 edition）to determine common peak. Cluster analysis were also conducted. RESULTS：A total of 12 common peaks were established in HPLC fingerprints of 13 batches of samples. The similarity was higher than 0.90. After validation,HPLC chromatograms of 13 batches of sample were in good agreement with control fingerprint. 13 batches of medicinal materials were clustered into 2 categories;S4,S6 and S10 were clustered into one category,and the rest were a category.CONCLUSIONS：Established fingerprint and cluster analysis results can provide reference for quality evaluation of H. platycladum and its exploitation and utilization.